摘要
本试验旨在研究不同磷源和水平及添加植酸酶对69~78周龄蛋鸡生产性能、蛋品质和骨代谢的影响,为高峰后蛋鸡饲粮选择合适磷源及磷添加水平提供理论依据。选取69周龄海兰褐蛋鸡2 475只,随机分为11个组,每组5个重复,每个重复45只鸡。对照组为玉米-豆粕型基础饲粮(不额外添加任何磷源),试验组分别以磷酸氢钙(DCP)[非植酸磷(NPP)添加水平分别为0.10%、0.15%、0.20%、0.25%和0.30%]和磷酸一二钙(MDCP)(NPP添加水平分别为0.07%、0.11%、0.15%、0.18%和0.22%)作为外源磷源,饲粮中均添加1 470 FTU/kg的植酸酶。试验预试期1周,正试期8周。结果表明:1)不同磷源和水平对69~78周龄蛋鸡的平均产蛋率、平均蛋重、料蛋比和破畸蛋率均无显著影响(P>0.05),但与对照组相比,以MDCP作为磷源能够显著提高平均日采食量(P<0.05)。2)与对照组和DCP组相比,以MDCP作为磷源显著提高了蛋黄颜色等级(P<0.05),且以0.18%NPP添加水平组的蛋黄颜色等级最高;与对照组相比,以DCP作为磷源对蛋黄颜色等级无显著影响(P> 0.05)。3)对照组蛋鸡血清甲状旁腺素(PTH)、护骨素(OPG)、Ⅰ型胶原C端肽(CTX-Ⅰ)含量以及抗酒石酸酸性磷酸酶(TRACP)活性均处于最高,且与对照组相比,0.15%、0.25%和0.30%NPP添加水平的DCP组血清PTH含量显著降低(P<0.05),0.15%~0.30%NPP添加水平的DCP组和0.11%~0.18%NPP添加水平的MDCP组血清OPG含量显著降低(P<0.05),0.15%~0.30%NPP添加水平的DCP组和0.11%~0.22%NPP添加水平的MDCP组血清TRACP活性显著降低(P<0.05);不同磷源和水平对蛋鸡血清降钙素(CT)、1,25-二羟基维生素D_(3)[1,25-(OH)_(2)-VD_(3)]、骨钙蛋白(BGP)和骨桥蛋白(OPN)含量以及骨碱性磷酸酶(BAP)活性均无显著影响(P> 0.05)。综上所述,在添加1 470 FTU/kg植酸酶的条件下,降低饲粮NPP水平不会对69~78周龄蛋鸡的产蛋率和料蛋比造成负面影响,但以MDCP作为磷源可提高蛋黄颜色等级;综合考虑生产性能、无机磷用量以及骨代谢健康等指标,高峰后蛋鸡饲粮建议以MDCP作为磷源,且适宜的NPP添加水平为0.11%(饲粮NPP水平为0.23%)。
This experiment was conducted to investigate the effects of different phosphorus sources and levels and phytase supplementation on performance,egg quality and bone metabolism of laying hens aged from 69 to78 weeks,and to provide theoretical basis for dietary phosphorus source selection and phosphorus supplemental level of laying hens after peak season. A total of 2 475 HyLine brown laying hens of 69-week-old were randomly divided into 11 groups with 5 replicates per group and 45 hens per replicate. Control group was fed a corn-soybean meal basal diet(without any additional phosphorus source),and the experimental groups were treated with calcium hydrogen phosphate(DCP) [non-phytate phosphorus(NPP)supplemental levels were0.10%,0.15%,0.20%,0.25% and 0.30%,respectively]and mono dicalcium phosphate(MDCP)(NPP supplemental levels were 0.07%,0.11%,0.15%,0.18% and 0.22%,respectively)as exogenous phosphorus sources. All diets were supplemented with 1 470 FTU/kg phytase. The pre-trial period lasted for 1 week,and the trial period lasted for 8 weeks. The results showed as follows:1)different phosphorus sources and levels had no significant effects on average laying rate,average egg weight,feed to egg ratio and abnormal egg rate of laying hens aged from 69 to 78 weeks(P>0.05). However,compared with the control group,MDCP as phosphorus source significantly increased the average daily feed intake(P<0.05). 2)Compared with the control group and DCP group,MDCP as phosphorus source significantly improved yolk color grade(P<0.05),and 0.18% NPP supplemental level group had the highest yolk color grade. Compared with the control group,DCP as phosphorus source had no significant effect on yolk color grade(P>0.05). 3)The contents of parathyroid hormone(PTH),osteoprotegerin(OPG)andⅠtype collagen C-terminal peptide(CTX-Ⅰ)as well as tartrate-resistant acid phosphatase(TRACP)activity in serum of laying hens in the control group were the highest,and compared with the control group,the serum PTH content in DCP groups supplemented with0.15%,0.25% and 0.30% NPP was significantly decreased(P<0.05),the serum OPG content in DCP group supplemented with 0.15% to 0.30% NPP and MDCP group supplemented with 0.11% to 0.18% NPP was significantly decreased(P<0.05),and the serum TRACP activity in DCP group supplemented with 0.15% to0.30% NPP and MDCP group supplemented with 0. 11% to 0. 22% NPP was significantly decreased(P <0.05). Different phosphorus sources and levels had no significant effects on serum contents of calcitonin(CT),1,25-dihydroxy vitamin D_(3) [1,25-(OH)_(2)-VD_(3) ],osteocalcin(BGP)and osteopontin(OPN)as well as bone alkaline phosphatase(BAP)activity of laying hens(P>0.05). In conclusion,under the condition of adding 1 470 FTU/kg phytase,reducing dietary NPP level has no negative effects on laying rate and feed to egg ratio of laying hens aged from 69 to 78 weeks,but MDCP as phosphorus source can improve yolk color grade. Considering performance,inorganic phosphorus consumption and bone metabolic health,it is recommended that MDCP be used as phosphorus source in the diet after peak season,and the appropriate NPP supplemental level is 0.11%(dietary NPP level is 0.23%).
作者
任跃昌
邓夕民
陈正平
单忠芳
刘亚萍
马璐璐
张鹏飞
万波扬
杨维仁
REN Yuechang;DENG Ximin;CHEN Zhengping;SHAN Zhongfang;LIU Yaping;MA Lulu;ZHANG Pengfei;WAN Boyang;YANG Weiren(Shandong Provincial Key Laboratory of Animal Bioengineering and Disease Control,College of Animal Science and Technology,Shandong Agricultural University,Tai’an 271018,China;Weifang Limin Feed Co.,Ltd.,Weifang 261000,China;Agricultural and Rural Center,Shazikou Street,Laoshan District,Qingdao City,Qingdao 266000,China;Jinan Spafas Poultry Co.,Ltd.,Ji’nan 250000,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2022年第6期3604-3614,共11页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
山东省重点研发计划(重大科技创新工程)项目“环境友好型猪禽无抗饲料和替抗养殖关键技术研究及应用”(2019JZZY020609)。
关键词
蛋鸡
磷源
磷水平
生产性能
蛋品质
骨代谢
laying hens
phosphorus source
phosphorus level
performance
egg quality
bone metabolism
