摘要
为优化超声辅助提取新疆管花肉苁蓉中苯乙醇苷的工艺,在单因素实验的基础上,取提取温度、乙醇浓度、料液比进行响应面优化,得到肉苁蓉苯乙醇苷的最佳提取工艺为:超声温度40℃,料液比1:41.7 g/mL、乙醇浓度51%,在该条件下苯乙醇苷得率为(14.36±0.12) mg/g。提取物主要由松果菊苷和毛蕊花糖苷构成,含量分别为(12.96±0.33) mg/g和(1.19±0.09) mg/g。苯乙醇苷提取物对羟基自由基和DPPH自由基具有较强的清除能力,其半抑制浓度(IC_(50))分别为60.196 mg/mL和0.875 mg/mL。本研究为新疆管花肉苁蓉苯乙醇苷的提取提供了基础数据。
In order to optimize the ultrasonic-assisted extraction process of phenylethanol glycosides from Cistanche tubulosa(Ct) in Xinjiang, box-behnken design was used to optimize the extraction conditions based on single factor experiments. Optimal conditions were the temperature of 40 ℃, the solid-liquid ratio of 1:41.7 g/mL and the ethanol concentration of 51%. The extraction yield under the optimal conditions was(14.36±0.12) mg/g. The extracts were mainly composed of echinacoside and verbascoside, the contents of which were(12.96±0.33) mg/g and(1.19±0.09) mg/g,respectively. The phenylethanoside extracts showed strong scavenging ability to hydroxyl radical and DPPH free radical,and the semi-inhibitory concentration(IC_(50)) values were 60.196 mg/mL and 0.875 mg/mL, respectively. This study provides basic data for the extraction of phenylethanoside from Cistanche tubulosa in Xinjiang.
作者
魏玉萍
赵岩
张丽
王琼琼
蒲云峰
侯旭杰
宋丽军
WEI Yuping;ZHAO Yan;ZHANG Li;WANG Qiongqiong;PU Yunfeng;HOU Xujie;SONG Lijun(College of Life Sciences,Tarim University,Alaer 843300,China;College of Bioengineering,Aksu Vocational and Technical College,Aksu 843000,China)
出处
《食品工业科技》
CAS
北大核心
2022年第13期148-155,共8页
Science and Technology of Food Industry
基金
南疆肉苁蓉精深加工关键技术及新产品创制(中央引导地方专项资金:兵财教2020-23号)
肉苁蓉黄酮低损制备及稳态化技术研究(新疆维吾尔自治区研究生科研创新项目XJ2021G294)。
关键词
管花肉苁蓉
苯乙醇苷
工艺优化
抗氧化活性
超声波辅助提取
Cistanche tubulosa
phenylethanoside
process optimization
antioxidant activity
ultrasonic-assisted extraction
