摘要
该实验的目的为研究干姜提取物对抗生素相关性腹泻(antibiotic-associated diarrhea,AAD)大鼠腹泻情况的改善及对肠道菌群的作用。使用抗生素混合溶液灌胃的方法建造AAD大鼠模型,使用培菲康70 mg·kg^(-1)·d^(-1)、干姜低剂量组200 mg·kg^(-1)·d^(-1)、干姜高剂量组400 mg·kg^(-1)·d^(-1)进行干预,观测大鼠体质量变化,通过粪便评分、120 min粪便质量、粪便含水量来评价大鼠的腹泻状况;HE染色大鼠结肠组织观察其病变程度;采用16S rRNA高通量测序技术来检测大鼠肠道菌群的变化。实验结果显示阳性药组、干姜低剂量组、干姜高剂量组经过对应药物治疗后与模型组相比,大鼠的粪便含水量、粪便评分及120 min粪便质量都有明显改善,通过结肠HE染色观察到结肠炎症情况明显好转,且干姜高、低剂量组治疗可以修复损伤的结肠组织。对肠道菌群分析结果显示,培菲康干预对AAD大鼠肠道菌群丰度和多样性无明显改善;高剂量干姜水煎液干预后,AAD大鼠肠道菌群的丰度显著增加,多样性也有明显上升趋势,且干姜高剂量组与阳性药组在菌群丰度和多样性上有显著性差异。在门水平上干姜干预后AAD大鼠厚壁菌门丰度提高,变形菌门丰度下降;在属水平上拟杆菌属、Lachnoclostridium、大肠杆菌-志贺菌等丰度下降;乳酸杆菌属、毛螺菌科等丰度上升。而培菲康干预后与AAD模型组相比肠道菌群无显著差异。该结果表明干姜对AAD具有较好疗效,并且能有效改善AAD大鼠肠道微生物环境。
This study was aimed to explore the effect of Zingiberis Rhizoma extract on rats with antibiotic-associated diarrhea(AAD),and reveal the modulation of gut microbiota during alleviation of AAD.AAD rat model was successfully established by exposing rats to appropriate antibiotic mixed solution.Peficon(70 mg·kg^(-1)·d^(-1))was used as positive control,then rats were treated with 200 mg·kg^(-1)·d^(-1)and 400 mg·kg^(-1)·d^(-1)of Zingiberis Rhizoma extract for low and high dosage groups of Zingiberis Rhizoma extract,respectively.The weight changes of the rats were observed,and the degree of diarrhea were evaluated by fecal score,120 min fecal weight and fecal water content.Colon tissues for histopathological examination were stained with hematoxylin and eosin(HE),and 16 S rRNA sequencing analysis of gut microbiota was performed.The results showed that compared with the model group,the degree of diarrhea,indicated by fecal water content,fecal score,and 120 min fecal weight of positive control group,Zingiberis Rhizoma low-dose group and Zingiberis Rhizoma high-dose group were significantly ameliorated.And the treatment of Zingiberis Rhizoma could significantly improve the pathological condition of colon tissue in AAD rats,especially the high dose of Zingiberis Rhizoma.In addition,16 S rRNA sequencing analysis of gut microbiota showed that the diversity and abundance of gut microbiota were significantly improved and the reco-very of gut microbiota was accelerated after given high-dose of Zingiberis Rhizoma,while no significant changes of alterations were observed after given Pefikon.Of note,compared with the pefikon group,the abundance and diversity of gut microbiota in Zingi-beris Rhizoma high-dose group were significantly elevated.At the phylum level,the abundance of Firmicutes in AAD rats increased and the abundance of Proteobacteria was decreased after the Zingiberis Rhizoma intervention.At the genus level,the abundance of Bacillus spp.,Lachnoclostridium and Escherichia coli-Shigella were decreased,and the abundance of Lactobacillus spp.,Trichophyton spp.,and Trichophyton spp.,etc.,were increased.While compared with the AAD model group,there was no significant difference of gut microbiota after given Peficon.The results showed that Zingiberis Rhizoma exerted beneficial health effects against AAD,and positively affected the microbial environment in the gut of rats with AAD.
作者
张学强
章从恩
于小红
马雨晴
李萌
段晓颖
马致洁
ZHANG Xue-qiang;ZHANG Cong-en;YU Xiao-hong;MA Yu-qing;LI Meng;DUAN Xiao-ying;MA Zhi-jie(Henan University of Chinese Medicine,Zhengzhou 450046,China;Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;the First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450008,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2022年第5期1316-1326,共11页
China Journal of Chinese Materia Medica
基金
中国博士后科学基金项目(2018M631704)
国家自然科学基金项目(82004032)。
关键词
干姜
抗生素相关性腹泻
肠道菌群
Zingiberis Rhizoma
antibiotic-associated diarrhea
gut microbiota
