摘要
采用3组实验饲料养殖225尾卵形鲳鲹(Trachinotus ovatus)幼鱼58 d,以不添加褐藻寡糖组(TC)作为对照,研究0.7 g·kg^(−1)(A1)和6.0 g·kg^(−1)(A2)褐藻寡糖对卵形鲳鲹生长、血浆生化及免疫指标、肝脏抗氧化能力、肠道形态和Nf-κb信号通路相关基因表达的影响。结果显示,A1和A2组增重率和特定生长率显著高于TC组(P<0.05),A1组饲料系数显著低于TC组(P<0.05);A1和A2组血浆补体C3质量浓度显著高于TC组(P<0.05),A2组碱性磷酸酶(AKP)活性显著高于TC组(P<0.05);与TC组相比,A1和A2组肝脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、谷胱甘肽还原酶(GR)活性和总抗氧化能力(T-AOC)显著升高,丙二醛(MDA)浓度显著降低(P<0.05);A1和A2组肠绒毛高度显著高于TC组(P<0.05);与TC组相比,A1和A2组ikk、nf-κb、tnf-α和il-8表达量显著降低,tgf-β表达量显著升高(P<0.05)。综上,添加褐藻寡糖可以改善卵形鲳鲹肠道形态和生长性能,提高血浆免疫指标和肝脏抗氧化能力,抑制肠道Nf-κb和促炎细胞因子mRNA的转录,并促进抗炎细胞因子mRNA的转录,建议卵形鲳鲹幼鱼饲料中褐藻寡糖的添加量为0.7 g·kg^(−1)。
Trachinotus ovatus juveniles(225 individuals)were fed for 58 d with three diets containing 0(Control:TC),0.7(A1)and 6.0 g·kg^(−1)(A2)alginate oligosaccharide(AO)to investigate the effects of AO on the growth,plasma biochemical and immune indexes,hepatic antioxidative capacity,intestine morphology and expression of genes involved in Nf-κb signaling pathway of T.ovatus.The results show that the weight gain rate and specific growth rate of T.ovatus of both A1 and A2 group were significantly higher than those of TC group(P<0.05).The feed coefficient of A1 group was significantly lower than that of TC group(P<0.05).Compared with TC group,the mass concentration of plasma C3 increased in both A1 and A2 group significantly(P<0.05),and the activity of alkaline phosphatase increased in A2 group significantly(P<0.05).Compared with TC group,the activities of SOD,CAT,POD and GR and T-AOC increased in both A1 and A2 significantly,while the concentration of MDA decreased significantly(P<0.05).The height of villus in A1 and A2 group were significantly higher than those of TC group(P<0.05).Compared with TC group,the expressions of ikk,nf-κb,tnf-αand il-8 decreased in A1 and A2 group significantly,while the expressions of tgf-βincreased significantly(P<0.05).In conclusion,AO supplementation in diet can improve the growth and intestine morphology of T.ovatus,enhance the plasma immune indexes and hepatic antioxidative capacity,inhibit the expression of intestinal Nf-κb and proinflammatory cytokine mRNA,and improve the expression of antiinflammatory cytokine mRNA.The suitable level of AO in diet is 0.7 g·kg^(−1).
作者
黄健彬
迟艳
周传朋
黄小林
黄忠
虞为
荀鹏伟
吴杨
张宇
林黑着
HUANG Jianbin;CHI Yan;ZHOU Chuanpeng;HUANG Xiaolin;HUANG Zhong;YU Wei;XUN Pengwei;WU Yang;ZHANG Yu;LIN Heizhao(College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306,China;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation&Utilization,Ministry of Agriculture and Rural Affairs,Guangzhou 510300,China;Wuzhoufeng Agricultural Science&Technology Co.Ltd.,Yantai 264000,China;Shenzhen Base of South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shenzhen 518121,China)
出处
《南方水产科学》
CAS
CSCD
北大核心
2022年第3期118-128,共11页
South China Fisheries Science
基金
中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金资助(2020TD55)
中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助(2021XK02)
深圳市科技计划项目(JCYJ20180306180203889)
广东省现代农业产业技术体系创新团队建设专项资金(2019KJ143)。
关键词
卵形鲳鲹
褐藻寡糖
生长性能
免疫
抗氧化能力
Trachinotus ovatus
Alginate oligosaccharide
Growth performance
Immunity
Antioxidative capacity
