摘要
为了解抗生素对石油降解菌的生态效应及微生物对典型抗生素毒害作用的应激响应机制,通过开展不同浓度典型抗生素对高效石油降解菌铜绿假单胞菌(ZS1)的毒性试验,研究了菌体细胞在3种不同种类抗生素(土霉素、红霉素、磺胺嘧啶)作用下的生长及毒理指标.结果表明,3种抗生素对ZS1有不同的抑菌效果,其中土霉素对ZS1活性的抑制程度最强,抑制率随着土霉素浓度的增大而明显上升,有显著的浓度-效应关系.研究了ZS1抗氧化酶(过氧化氢酶、过氧化物酶、超氧化物歧化酶)对抗生素的响应机制,结果表明ZS1受到污染时会通过产生抗氧化酶抵抗外界污染并维持细胞功能.
In order to elucidate the ecological effects of antibiotics on oil-degrading bacteria and the stress response mechanisms of microorganisms to the toxic effects of typical antibiotics,toxicity tests of different concentrations of typical antibiotics on oil-degrading bacteria pseudomonas aeruginosa(ZS1)were carried out,and the growth and toxicity of thallus cells treated with three kinds of antibiotics(Oxytetracycline,Erythromycin and Sulfadiazine)were studied.The results showed that three antibiotics had different bacteriostatic effects on ZS1,among which Oxytetracycline had the strongest inhibitory effect on ZS1 activity,and the inhibition rate was increased obviously with increasing Oxytetracycline concentration,and there was a significant concentration-effect relationship.The mechanism of ZS1 antioxidant enzymes(Catalase,Peroxidase and Superoxide dismutase)in response to antibiotics was investigated.The results showed that ZS1 could resist external pollution and maintain cellular function by producing antioxidant enzymes when it was contaminated.
作者
刘苏翔
高宇
乔延路
薛建良
Liu Suxiang;Gao Yu;Qiao Yanlu;Xue Jianliang(College of Safety and Environmental Engineering,Shandong University of Science and Technology,Qingdao,Shandong 266590)
出处
《石油炼制与化工》
CAS
CSCD
北大核心
2022年第5期115-122,共8页
Petroleum Processing and Petrochemicals
基金
国家自然科学基金项目(42106151)。
关键词
抗生素
石油降解菌
生长抑制率
抗氧化酶
应激响应机制
antibiotic
petroleum degrading bacterial
growth inhibition ratio
antioxidant enzymes
responding mechanism
