摘要
近年来,水体镉污染日益严峻,筛选超富集植物用于其治理具有重要意义。本研究以90个浮萍株系为实验材料,采用30 mg·L^(-1)的镉处理7 d,获得7个镉耐受优势株系。通过Blast比对和构建系统发育树鉴定该7个浮萍株系的种属,确定为Spirodela polyrhiza、Lemna japonica、Lemna minor和Landoltia punctata。通过低质量浓度(0.5 mg·L^(-1))和高质量浓度(10 mg·L^(-1))镉处理7 d,进一步比较和研究了7个优势株系对镉的富集效果。结果表明,低浓度镉处理后,5个株系的镉富集量超过100 mg·kg^(-1);高浓度镉处理后,7个浮萍株系的镉富集量超过1200 mg·kg^(-1),生物富集系数大于120,对水体中镉的去除率高于70%。其中,4号株系(L.japonica)为本次筛选出的最佳株系,其镉富集量、生物富集系数和对水体中镉的去除率分别达到2834.30 mg·kg^(-1)、283.43和82.50%。
In recent years,the cadmium pollution in water has become severe,and it is of great significance to screen hyperaccumulators for its treatment.In this study,90 duckweed strains were taken as experimental materials and treated with 30 mg·L^(-1) cadmium for 7 days,then 7 cadmium-tolerant dominant strains were obtained.Through Blast comparison and construction of phylogenetic trees,the species of these 7 duckweed strains were identified as Spirodela polyrhiza,Lemna japonica,Lemna minor and Landoltia punctata.After treatment with low concentration(0.5 mg·L^(-1))and high concentration(10 mg·L^(-1))cadmium for 7 days,the enrichment effects of 7 dominant strains were further studied and compared.Under low concentration cadmium treatment,the cadmium concentration of 5 duckweed strains exceeded 100 mg·kg^(-1).Under high concentration cadmium treatment,the cadmium concentration of 7 duckweed strains exceeded 1200 mg·kg^(-1).The bioconcentration factors were higher than 120,and the water cadmium removal rates were higher than 70%.Among them,4 duckweed strain(L.japonica)was the best strain selected in this study,and its cadmium concentration,bioconcentration factor and water cadmium removal rate reached 2834.30 mg·kg^(-1),283.43 and82.50,respectively.
作者
郑萌盟
谭艾娟
吕世明
冯丹
杨潇
罗菲
杨贵利
ZHENG Mengmeng;TAN Aijuan;LV Shiming;FENG Dan;YANG Xiao;LUO Fei;YANG Guili(Key laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Guizhou Key Lab of Agro-Bioengineering,College of Life Sciences/Institute of Agro-bioengineering,Guizhou University,Guiyang 550025,China;College of Animal Science,Guizhou University,Guiyang 550025,China;State Key Laboratory of Environmental Geochemistry,Institute of Geochemistry,Chinese Academy of Sciences,Guiyang 550025,China)
出处
《环境工程学报》
CAS
CSCD
北大核心
2022年第2期471-480,共10页
Chinese Journal of Environmental Engineering
基金
国家自然科学基金青年资助项目(32001203)
国家自然科学基金地区科学基金资助项目(31760749)
贵州省科技支撑计划项目(黔科合支撑[2018]2276)
贵州大学人才引进项目(贵大人基合字[2019]60)。
关键词
浮萍
重金属镉
品种鉴定
超富集植物
水体污染
duckweed
heavy metal cadmium
species identification
hyperaccumulators
water pollution
