摘要
在高53 cm、底36 cm和顶部直径48 cm的聚乙烯筒内注入过滤后曝气3 d的自来水60 L,筒底中间放置发射方向朝上的40 W超声波探头,试验玻璃容器悬浮于水面,距离超声波发射探头30 cm,内放铜绿微囊藻Microcystis aeruginosa FACHB-924和铜绿微囊藻FACHB-905,起始OD560分别为0.305和0.364,在第0 d、1 d、2 d、4 d、6 d和8 d依次测量两种不同株系悬浮铜绿微囊藻的密度。试验组和对照组筒中均饲养鲫Carassius auratus 25尾,铜锈环棱螺Bellamya aeruginosa 15个(3组500 mL烧杯,每组5个),克氏原螯虾Procambarus clarkia 9只(1 L烧杯中分三组,每组3只);大型蚤Daphnia magna的起始密度分别为160 ind./L和137 ind./L;长势良好狐尾草Alopecurus pratensis和浮萍Lemna mino培养14 d,比较试验组和对照组浮萍叶片分裂数目及狐尾草鲜重、分枝数目、总长度及根须数目增加百分比,研究低强度超声波控制铜绿微囊藻的效果及对渔业环境安全的影响。结果显示:3 d后大型蚤种群密度增加了5.23倍;8 d后水体中悬浮铜绿微囊藻生物量比初始值分别降低了89.01%和83.05%;铜锈环棱螺、克氏原螯虾、鲫的存活未受影响;浮萍叶片数目有所增加,狐尾草分枝数目、湿重、整体长度、根须数目分别增加了7.09%、17.11%、37.13%和43.49%。低强度超声波显著控制了水体中悬浮铜绿微囊藻总量,对水生动物、水生植物及浮游动物安全的影响不显著。
Bluegreen alga strains of Microcystis aeruginosa FACHB-924 and M.aeruginosa FACHB-905 in a glass container suspended on the water surface and at distance from an ultrasonic emission probe 30 cm were disposed at the initial OD560 values of 0.305 and 0.364 in a polyethylene cylinder with a height of 53 cm and a diameter of 36 cm and 48cm at the bottom and top,respectively,installed a 40 W ultrasonic transmitter with the probe facing upward in the middle of the bottom of the cylinder filled with 60 L of filtered and aeration tap water for 3 d.The suspension densities of the bluegreen alga strains were determined 0 d,1 d,2 d,4 d,6 d and 8 d after the administration.Meanwhile 25 individuals of crucian carp(Carassius auratus),15 individuals of snail(Bellamya aeruginosa)(a 500 mL beaker separated into 3 parts,5 in each parts),and 9 individuals of red swamp crayfish(Procambarus clarkii)were reared in the polyethylene cylinder,together with water fleas(Daphnia magna)at initial density of 160 ind./L and 137 ind./L,foxtail grass(Alopecurus pratensis)and duckweed(Lemna mino)for 14 d,to compare the number of reproductive leaves of the duckweed and the percentage increase in fresh weight,number of branches,total length and number of roots of duckweed in the test group and the control group on 0 d and 14 d,in order to evaluate the effects of low intensity ultrasonic wave on the bluegreen alga control and on fishery environmental safety.The results showed that the biomass of the suspended bluegreen alga was shown to be reduced by 89.01%and 83.05%compared with the initial value after eight days culture.The density of the water fleas was increased by 5.23 times after three days,without significant changes in the survival of the snail,red swamp crayfish and crucian carp.The number of leaves on the duckweed was increased,and there was increased by 7.09%in the number of branches,by 17.11%in wet weight,by 37.13%in overall length and 43.49%amounts in root tubers of the foxtail grass.The finding revealed that low intensity ultrasonic wave significantly controlled the total amount of suspended bluegreen alga in water,suitable for control of outbreaks of cyanobacteria bloom in aquaculture waters without no obvious effect on aquatic animals,aquatic plants and zooplankton.
作者
邵泽宇
陆开宏
谢吉国
杨显祥
董小敬
杜雪地
王曙光
吴飞
SHAO Zeyu;LU Kaihong;XIE Jiguo;YANG Xianxiang;DONG Xiaojing;DU Xuedi;WANG Shuguang;WU Fei(Yangzhou Fisheries Technology Extension Center,Yangzhou 225000,China;Ningbo Department of Ocean and Fisheries,Ningbo 315000,China;Beijing City Daxing Fisheries Technology Extension Center,Beijing 102600,China;College of Animal Science and Technology,Yangzhou University,Yangzhou 225000,China)
出处
《水产学杂志》
CAS
北大核心
2021年第1期68-72,共5页
Chinese Journal of Fisheries
基金
江苏现代农业产业技术体系建设项目(JATS-2019-254).
关键词
低强度超声波
铜绿微囊藻
渔业环境
安全性
low intensity ultrasound
Microcystis aeruginosa
fishery environment
risk
