摘要
目的建立GC-MS同时测定复方鲜竹沥液中苯酚、愈创木酚、甲基愈创木酚、乙基愈创木酚、乙烯基愈创木酚、2,6-二甲氧基苯酚、香兰素和丁香醛8个酚类有效成分含量的方法。方法样品经乙醚萃取后,采用DB-35MS UI色谱柱(30 m×0.25 mm,0.25μm)进行分离,采用离子监测模式(SIM)进行质谱检测,外标法定量。结果在优化实验条件下,8种酚类成分分离良好,在0.1~129.4μg·mL^(-1)内线性良好,相关系数均>0.9990,方法检出限均<0.043μg·mL^(-1);在3个不同加标浓度下的回收率为93.76%~101.33%,RSD值均≤3.08%。结论该方法简单、灵敏、准确并且可靠,可为复方鲜竹沥液的质量控制提供科学依据。
OBJECTIVE To establish a GC-MS method for the simultaneous determination of 8 phenolic active components such as phenol,guaiacol,2-methoxy-4-methylphenol,4-ethyl-2-methoxy-phenol,4-hydroxy-3-methoxy-styrene,2,6-dimethoxyphenol,vanillin and syringaldehyde in Fufang Xianzhuli Ye.METHODS The samples were extracted with ether and separated by a DB-35MS UI column(30 m×0.25 mm,0.25μm),then the extract was detected by MS under SIM mode.And external standard method was used for quantification.RESULTS Under the optimized experimental conditions,8 phenolic components were well separated.The linearity was good within the mass concentration range of 0.1-129.4μg·mL^(-1),correlation coefficients were>0.9990.The detection limits of phenolic active components were<0.043μg·mL^(-1).The spiked recoveries were in the range of 93.76%-101.33% with RSD≤3.08%.CONCLUSION The method is simple,sensitive,accurate and reliable,and can provide some scientific basis for quality control of Fufang Xianzhuli Ye.
作者
肖小武
刘静佳
周志强
许妍
罗跃华
付辉政
洪挺
XIAO Xiaowu;LIU Jingjia;ZHOU Zhiqiang;XU Yan;LUO Yuehua;FU Huizheng;HONG Ting(Jiangxi Provincial Institute for Drug Control,NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Medicine,Jiangxi Provincial Engineering Research Center for Drug and Medical Device Quality,Nanchang 330029,China;Nanchang Institute of Technology,Nanchang 330044,China)
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2022年第6期788-793,共6页
Chinese Journal of Modern Applied Pharmacy
基金
江西省重点研发计划项目(20203BBG73062)
江西省药品监督管理局科研项目(2020JS04)。
