摘要
目的观察参地颗粒对C-BSA大鼠肾组织中miRNA-193a和WT-1 mRNA的干预作用。方法40只雄性SD大鼠随机分为正常组、模型组、参地颗粒组、缬沙坦组各10只。参地颗粒组给予参地颗粒水溶液4.0 g/(kg·d)灌胃,缬沙坦组给予缬沙坦胶囊混悬液20 mg/(kg·d)灌胃,正常组、模型组予生理盐水灌胃,每次3 mL,每天1次,连续给药8周后处理动物、留取尿液和肾脏标本。酶联免疫吸附法(ELISA)检测尿白蛋白和尿肌酐浓度、尿Nephrin和Podocalyxin含量,实时荧光定量逆转录聚合酶链反应(qRT-PCR)检测肾组织中WT-1及miRNA-193a mRNA表达。透射电镜观察肾小球超微结构图,并计算肾小球基底膜(GBM)平均厚度(TGBM)。结果与正常组比较,模型组大鼠的尿白蛋白/尿肌酐比值(UACR)、尿Nephrin和Podocalyxin含量、肾组织miR-193a mRNA表达量、TGBM均显著升高,而WT-1 mRNA表达量显著减少(P<0.05)。与模型组比较,参地颗粒组和缬沙坦组大鼠的UACR、尿Nephrin和Podocalyxin含量、肾组织miR-193a mRNA表达量、TGBM均显著下降,WT-1 mRNA表达量显著增加(P<0.05);且参地颗粒组的UACR、尿Podocalyxin、miR-193a和WT-1 mRNA表达量、TGBM变化均显著优于缬沙坦组(P<0.05)。正常组肾小球足细胞结构正常,模型组足突可见大部分融合或消失,基底膜弥漫性增厚,上皮下可见大块电子致密物沉积。参地颗粒组和缬沙坦组的足细胞结构较模型组有所改善。结论参地颗粒可能通过抑制C-BSA大鼠肾组织中miR-193a表达,并上调WT-1表达,进而减轻足细胞损伤,发挥降低尿蛋白作用。
Objective To observe the intervention effect of Shendi granule on miRNA-193a and WT-1mRNA in renal tissue of C-BSA rats.Methods Forty male SD rats were randomly divided into normal group(n=10),model group(n=10),Shendi granule group(n=10)and valsartan group(n=10).The rats in the Shendi granule group were given intragastric administration of Shendi granule aqueous solution 4.0 g/(kg·d),the valsartan group was given valsartan capsule suspension20 mg/(kg·d),and the normal group and model group were given normal saline,once a day,once a day.After 8 weeks of continuous administration,the animals were treated,urine and kidney samples were collected.The concentrations of urinary albumin and creatinine,the contents of urinary Nephrin and Podocalyxin were detected by enzyme-linked immunosorbent assay(ELISA),and the expression of WT-1 and miRNA-193a mRNA in renal tissue was detected by real-time fluorescence quantitative reverse transcriptase polymerase chain reaction(qRT-PCR).The ultrastructure of glomeruli was observed by transmission electron microscope,and the average thickness of glomerular basement membrane(GBM)(TGBM)was calculated.Results Compared with the normal group,the ratio of urinary albumin to creatinine(UACR),the contents of urinary Nephrin and Podocalyxin,the expression of miR-193amRNA and TGBM in kidney tissue were significantly increased,while the expression of WT-1mRNA was significantly decreased in the model group.Compared with the model group,the contents of UACR,urinary Nephrin and Podocalyxin,the expression of miR-193amRNA and TGBM in kidney tissue were significantly decreased,and the expression of WT-1mRNA was significantly increased in Shendi granule group and valsartan group,and the changes of UACR,urinary Podocalyxin,miR-193a and WT-1mRNA expression and TGBM in Shendi granule group were significantly better than those in valsartan group(P<0.05).The structure of glomerular podocytes in the normal group was normal.In the model group,most of the foot processes fused or disappeared,the basement membrane thickened diffusely,and large electronic dense deposits could be seen under the epithelium.The podocyte structure of Shendi granule group and valsartan group was better than that of model group.Conclusion Shendi granule may reduce podocyte injury and reduce urinary protein by inhibiting the expression of miR-193a and up-regulating the expression of WT-1 in renal tissue of C-BSA rats.
作者
项建军
金华
韩佳月
王亿平
XIANG Jianjun;JIN Hua;HAN Jiayue;WANG Yiping(The First Affiliated Hospital of Anhui Traditional Chinese Medicine University,Hefei 230038,China;Department of Nephrology,the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine,Hefei 230031,China)
出处
《云南中医学院学报》
2021年第3期11-16,共6页
Journal of Yunnan University of Traditional Chinese Medicine
基金
安徽省高校自然科学研究重点项目(KJ2019A0441)
2021安徽省医疗重点专科项目
2016年安徽省中医发展专项资金:王亿平名中医工作室(卫办秘[2016]680号)
安徽中医药领军人才项目(中医药发展秘[2018]23号)。
