摘要
目的观察参地颗粒对系膜增生性肾小球肾炎(Ms PGN)大鼠T细胞亚群及足细胞标志蛋白的影响,探讨参地颗粒的作用机制。方法建立Ms PGN模型,将40只SD大鼠随机分为模型组、缬沙坦组、参地颗粒组和正常组。参地颗粒组按4 g/(kg·d)灌胃,缬沙坦组按10.3 mg/(kg·d)灌胃,模型组及正常组每日给予等量温水灌胃,连续灌胃12周。常规生化法检测各组大鼠24 h尿蛋白定量、流式细胞术检测全血CD4+T细胞、CD8+T细胞数量,ELISA检测血清白细胞介素2(IL-2)、IL-4、IL-17和尿足萼糖蛋白(PCX)、B7-1水平及肾脏组织中钙调磷酸酶(Ca N)含量。结果模型组24 h尿蛋白定量、CD8+T细胞水平,血清IL-2、IL-17和尿PCX、B7-1水平及肾脏组织中Ca N含量均高于正常组;缬沙坦组和参地颗粒组以上指标均低于模型组,参地颗粒组优于缬沙坦组。模型组全血CD4+T细胞及血清IL-4水平均低于正常组,缬沙坦组和参地颗粒组以上指标均高于模型组,参地颗粒组优于缬沙坦组。结论参地颗粒可有效降低Ms PGN大鼠24 h尿蛋白定量,可能与增加CD4+T细胞数量,降低血清IL-2、IL-17及尿PCX、B7-1水平和肾脏组织中Ca N的表达,增加血清IL-4水平有关。
Objective To observe the effect of Shendi granules on T cell subsets and podocyte marker protein in rats with mesangial proliferative glomerulonephritis（ Ms PGN）,and study possible mechanism. Methods Totally 40 SD rats were randomly divided into the model group,valsartan group,Shendi granule group and normal group. The Shendi granule group were given 4 g/（ kg·d） of Shendi granule by gavage; the valsartan group were given 10. 3 mg/（ kg · d） of valsartan by gavage; the model group and normal group were given the same amount of saline per day by gavage. The treatments lasted12 weeks. Routine biochemical method was used to quantify 24-hour urine protein; the numbers of CD4~＋and CD8~＋T cells were detected by flow cytometry; the serum levels of interleukin 2（ IL-2）,IL-4 and IL-17,the levels of urinary podocalyxin（ PCX） and B7-1,the renal calcineurin（ Ca N） content were determined by ELISA. Results Compared with the normal group,the levels of 24-hour urine protein,CD8~＋T cells,serum IL-2 and IL-17,urinary PCX and B7-1,CaN in the model group were higher. The above indexes in the valsartan group and control group were lower than those in the model group,they were lower in the Shendi granule group than in the valsartan group. The whole blood CD4~＋T cell number and serum IL-4 level in the model group were lower than those in the normal group,they were higher in the valsartan group and control group than in the model group. Compared with the valsartan group,the Shendi granule group had a better improvement.Conclusion Shendi granule could reduce 24-hour urine protein effectively. The mechanism may be related to the regulation of CD4~＋T and CD8~＋T cell numbers,the down-regulated expressions of serum IL-2,IL-17,the decreased levels of PCX and B7-1 in urine,CaN in kidney tissue,and the up-regulated level of serum IL-4.
作者
王亿平
张磊
王东
魏玲
金华
章雪莲
胡岚
WANG Yiping ZHANG Lei WANG Dong WEI Ling JIN Hua ZHANG Xuelian HU Lan(First Affiliated Hospital, Anhui University of TCM, Hefei 230031, China)
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2017年第8期1014-1019,共6页
Chinese Journal of Cellular and Molecular Immunology
基金
安徽省卫生厅中医药科研项目(2012ZY13)
关键词
系膜增生性肾小球肾炎
参地颗粒
T细胞亚群
足细胞
mesangial proliferative glomerulonephritis
Shendi granule
T cell subsets
podocytes
