摘要
目的 基于网络药理学探索葛根-丹参药对治疗冠心病心绞痛的作用机制。方法 借助多个在线数据库筛选并预测葛根-丹参药对活性成分及治疗治冠心病心绞痛的作用靶标。借助STRING数据库构建靶蛋白相互作用(PPI)网络;通过Uniprot数据库获取靶标蛋白的基因名称,利用DAVID在线工具进行潜在基因的GO分析及KEGG富集通路分析。结果从TCMSP数据库筛选出葛根-丹参药对的69种活性成分。PPI网络中包含72个蛋白,涉及IL6、FOS、CASP3、EGFR等关键蛋白。GO富集分析得到GO条目112个,以分子功能为主。KEGG富集通路分析得到119条信号通路。结论 葛根-丹参药对主要通过调控炎症反应、细胞增殖凋亡等途径发挥治疗冠心病心绞痛的作用。
Objective To explore the mechanism of radix puerariae-salvia miltiorrhiza couplet medicines in the treatment of angina pectoris of coronary heart disease based on network pharmacology. Methods The active components of radix puerariae-salvia miltiorrhiza couplet medicines and its therapeutic targets for coronary heart disease with angina pectoris were screened and predicted from multiple online databases. The STRING database was used to construct a protein-protein interaction network(PPI). The gene name of the target protein was obtained by the Uniprot database,the gene ontology(GO)analysis of potential genes and KEGG pathway analysis were carried out by using DAVID online tool. Results The 69 active components of radix puerariae-salvia miltiorrhiza couplet medicines were screened from the TCMSP database. The PPI network contained 72 proteins,and the key proteins are involved in IL6,FOS,CASP3,EGFR,etc. GO analysis obtained 112 GO entries,mainly based on molecular function. The KEGG pathway enrichment analysis obtained 119 signaling pathways. Conclusion Radix puerariae-salvia miltiorrhiza couplet medicines play a role in the treatment of coronary heart disease with angina pectoris by participating in the regulation of the inflammatory response,cell proliferation and apoptosis.
作者
阙斌
常佩芬
周红梅
冯思婷
王晓
郭倩
聂绍平
QUE Bin;CHANG Peifen;ZHOU Hongmei;FENG Siting;WANG Xiao;GUO Qian;NIE Shaoping(Center for Coronary Artery Disease,Beijing Anzhen Hospital Affiliated to Capital Medical University,Beijing 100029,China;Department of Cardiology,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China;Beijing Institute of Heart,Lung and Blood Vessel Diseases,Beijing 100029,China)
出处
《中国中医药现代远程教育》
2022年第7期132-134,共3页
Chinese Medicine Modern Distance Education of China
基金
北京市中医药管理局北京市中医药科技发展资金项目[No.JJ2018-01]。
关键词
网络药理学
葛根-丹参药对
胸痹
冠心病心绞痛
network pharmacology
radix puerariae-salvia miltiorrhiza couplet medicines
chest discomfort
angina pectoris of coronary heart disease
