摘要
以四川省普格县野生微香菇属真菌(Lentinula)子实体为试材,采用组织分离的方法得到菌株HG-2、BG-1,并对其进行分子鉴定;同时研究了不同碳源、氮源、温度以及pH对HG-2、BG-1菌株菌丝生长的影响,以期为野生食用菌资源的开发利用提供参考依据。结果表明:2个野生菌株均为微香菇属(Lentinula edodes)真菌。HG-2、BG-1菌株菌丝体生长的最佳碳源为玉米粉,最佳氮源为酵母膏,最适培养温度为25℃,最适pH为6.0。
Taking the fruiting bodies of wild Lentinula fungus collected from the mountain in Puge county,Sichuan Province as the test material,strains HG-2 and BG-1 were obtained by tissue isolation.Strains HG-2 and BG-1 were identified by DNA sequences.The effects of different carbon sources,nitrogen sources,temperature and pH on the growth of HG-2 and BG-1 strains were studied,in order to provide a reference for the utilization of wild Lentinula resources.The results showed that the two wild strains were Lentinula edodes.The optimum carbon source for the mycelial growth of HG-2 and BG-1 strains was corn meal,the optimum nitrogen source was yeast extract;the optimal cultivation temperature was 25℃,and the optimal pH was 6.0.
作者
方志荣
清源
何晓兰
乃戈日优
FANG Zhirong;QING Yuan;HE Xiaolan;NAIGE Riyou(Resource and Environment Department,Xichang University,Xichang,Sichuan 615013;Panxi Crops Research and Utilization Key Laboratory of Sichuan Province,Xichang University,Xichang,Sichuan 615013;Soil and Fertilizer Institute,Sichuan Academy of Agricultural Sciences,Chengdu,Sichuan 610066;Education,Sports and Science and Technology Bureau of Puge County,Puge,Sichuan 615302)
出处
《北方园艺》
CAS
北大核心
2022年第6期107-113,共7页
Northern Horticulture
基金
凉山彝族自治州科学技术局资助项目(17NYCX0023)
攀西特色作物研究与利用四川省重点实验室资助项目(XNFZ2109)。
关键词
野生香菇
ITS序列分析
生物学特性
Lentinula edodes
ITS sequence analysis
biological characteristic
