摘要
[目的]探讨ABCE1乙酰化与乙酰基转移酶KAT5、p300/CBP及PCAF在食管癌中的表达相关性及其临床意义。[方法]选择70例食管鳞癌组织及其癌旁食管黏膜组织,采用免疫共沉淀检测组织中ABCE1乙酰化水平,qRT-PCR检测KAT5、p300/CBP及PCAF组织中mRNA相对表达量,免疫组织化(SP)和Western-blot检测蛋白表达,结合临床病理资料进行相关性分析。[结果 ]食管癌组织中ABCE1乙酰化率为65.34%±2.42%,高于正常食管黏膜的14.25%±1.15%(P<0.05);癌组织中KAT5与p300/CBP mRNA相对表达量为4.48±1.03和3.75±0.84,高于正常食管黏膜的1.13±0.14和1.44±0.37(P<0.05);癌组织中PCAF mRNA相对表达量为1.02±0.26,低于正常食管黏膜中的3.56±0.73(P<0.05);癌组织中KAT5、p300/CBP与PCAF的阳性表达率分别为75.71%(53/70)、68.57%(48/70)和24.28%(17/70),与正常食管黏膜的18.57%(13/70)、12.86%(9/70)和65.71%(46/70)相比,差异均有统计学意义(P<0.05)。Western-blot结果显示,癌组织中KAT5、p300/CBP与PCAF的蛋白表达量分别为78.46%±4.14%、67.48%±3.78%和7.17%±1.46%,与正常食管黏膜的34.75%±2.38%、28.74%±2.15%和57.49%±2.47%相比,差异均有统计学意义(P<0.05);ABCE1乙酰化、KAT5、p300/CBP与PCAF的阳性表达与TNM分期、肿瘤分化程度及淋巴结转移密切相关(P<0.05)。食管癌中ABCE1乙酰化与PCAF的表达呈负相关,与KAT5和p300/CBP的表达呈正相关。[结论] ABCE1乙酰化与乙酰基转移酶PCAF、KAT5及p300/CBP的表达密切相关,其KAT5及p300/CBP高表达可能是催化ABCE1发生乙酰化的因子并促进了食管癌的发生和发展。
[Objective] To investigate the correlation and clinical significance of ABCE1 acetylation and KAT5, p300/CBP and PCAF expression in esophageal cancer.[Methods] Seventy cases esophageal squamous carcinoma tissue and normal esophageal mucosal tissue were selected. The ABCE1 acetylation levels in the tissues were detected by immunoprecipitation. The relative mRNA expression of PCAF, KAT5 and p300/CBP were detected by qRT-PCR, and the protein expression was detected by immunohistanization(SP) and Western-blot. Correlation analysis was combined with clinicopathological data. [Results] ABCE1 ethylation rate was 65.34% ±2.42% in esophageal cancer tissue, higher than that in normal esophageal mucosa tissue14.25% ±1.15%(P <0.05).The relative mRNA expression of KAT5 and p300/CBP was 4.48 ±1.03 and 3.75 ±0.84 in cancer tissue, higher than those in normal esophageal mucosa tissue 1.13±0.14 and 1.44±0.37(P<0.05). The relative expression of PCAF mRNA in cancer tissue was 1.02 ±0.26, lower than that in normal esophageal mucosa tissue 3.56±0.73(P<0.05). Positive expression rates of KAT5, p300/CBP and PCAF in cancer tissues were 75.71%(53/70), 68.57%(48/70) and 24.28%(17/70), and in the normal esophageal mucosa tissue were 18.57%(13/70), 12.86%(9/70) and 65.71%(46/70)(all P<0.05).Western-blot results show that the protein expression of KAT5, p300/CBP and PCAF in cancerous tissues was 78.46% ±4.14%, 67.48%±3.78% and 7.17%±1.46%, there were significant differences compared with those in normal esophageal mucosa(34.75%±2.38%, 28.74%±2.15%, 57.49%±2.47%, P<0.05). ABCE1 acetylation, positive expression of KAT5, p300/CBP and PCAF were closely associated with TNM staging, tumor differentiation, and lymph node metastasis(P<0.05). The acetylation of ABCE1 was negatively associated with PCAF expression and positive correlation with the expression of KAT5 and p300/CBP in the esophageal cancerous tissues. [Conclusion] ABCE1 acetylation is closely related to the expression of acetyltransferases PCAF, KAT5 and p300/CBP, the higher expression of KAT5 and p300/CBP may be factors catalyze acetylation and promote the occurrence and development of esophageal cancer.
作者
梁宗英
张乐
赵宝山
侯继申
辛国华
LIANG Zong-ying;ZHANG Le;ZHAO Bao-shan;HOU Ji-shen;XIN Guo-hua(The Affiliated Hospital of Chengde Medical College,Chengde 067000,China)
出处
《肿瘤学杂志》
CAS
2022年第2期122-127,共6页
Journal of Chinese Oncology
基金
河北省医学科学研究重点课题计划项目(20181159)。
