摘要
目的采用网络药理学方法研究银杏叶治疗心绞痛(angina pectoris,AP)的生物机制。方法从中药系统药理学分析平台(TCMSP)筛选银杏叶的化学活性成分,收集作用靶标;采用CTD、TTD、Drugbank、OMIM和Gene Cards数据库检索AP相关靶点。采用Cytoscape软件构建成分-靶点-疾病网络图,利用STRING数据库构建靶蛋白相互作用网络图,并对相关靶点进行GO功能富集分析、KEGG信号通路分析。结果筛选后获得银杏叶有效成分30个,治疗AP的潜在靶点基因111个。有效成分槲皮素、山柰素、阿魏酸等,主要作用于PIK3CG、MAPK14、NOS3、AKT1等关键靶点,通过流体剪切应力动脉粥样硬化、PI3K-Akt、MAPK、钙信号通路等通路,发挥抗炎、抗氧化、抗凋亡、调节离子通道等功能治疗AP。结论利用中药"多成分-多靶点-多通路"的特点,探究银杏叶治疗AP的潜在作用机制,为银杏叶治疗AP以至扩大适应证的后续科学研究及临床应用提供了一定参考。
Objective To explore the biological mechanism by which Ginkgo Biloba protects against angina pectoris(AP)using network pharmacology. Methods The chemical active ingredients of Ginkgo Biloba leaves were screened from the Traditional Chinese Medicine System Pharmacology Analysis Platform(TCMSP) and the targets were collected. Such databases as TTD, TTTD, Drugbank, OMIM and GeneCards were searched for AP-related targets. A component-target-disease network was constructed with Cytoscape software. The protein-protein interaction(PPI) network was analyzed using STRING database.GO enrichment analysis and KEGG signal pathway analysis were performed on related targets. Results After selection, 30 active components of Ginkgo Biloba leaves and 111 potential target genes for AP were obtained. The active ingredients, such as quercetin, kaanide and ferulic acid, mainly acted on PIK3 CG, MAPK14, NOS3, Akt1 and other key targets. Through fluid shear stress, PI3 K-Akt, MAPK, calcium signaling pathway and other signaling pathways, these active ingredients protected against angina pectoris by means of anti-inflammation, anti-oxidation, anti-apoptosis, and regulation of ion channels. Conclusion The potential mechanism of Ginkgo Biloba for AP has been explored based on the multi-component, multi-target, and multipathway characteristics of traditional Chinese medicine, which can facilitate subsequent research and clinical application of Ginkgo Biloba in the treatment of AP and extended indications.
作者
张强
杜鹏程
满春蕊
孙倩
ZHANG Qiang;DU Pengcheng;MAN Chunrui;SUN Qian(Dezhou People’s Hospital,Dezhou Shandong 253000,China)
出处
《中国药物警戒》
2022年第2期158-163,共6页
Chinese Journal of Pharmacovigilance
基金
山东省医药卫生科技发展计划(2016WS0070)。
关键词
银杏叶
心绞痛
网络药理学
蛋白相互作用
富集分析
Ginkgo Biloba
angina pectoris
network pharmacology
protein-protein interaction
enrichment analysis
