摘要
目的建立超高效液相色谱(UPLC)柱前衍生法测定滇重楼中15种氨基酸含量,并比较分析不同产地的野生与栽培滇重楼氨基酸含量差异。方法以6 mol·L;盐酸溶液110℃水解22 h,衍生试剂为异硫氰酸苯酯,测定3个省27批滇重楼中15种氨基酸的含量。采用SPSS 20.0软件对其氨基酸的含量进行t检验。结果质量浓度在0.354~30.912μg·mL^(-1)范围内的15种氨基酸线性关系良好(r=0.999 7~0.999 9);平均回收率为95.12%~101.05%,RSD为1.32%~2.82%,检出限为0.031~0.089μg·mL^(-1),定量限为0.104~0.297μg·mL^(-1)。3个省27批滇重楼均以天门冬氨酸、精氨酸和谷氨酸含量较高;滇重楼栽培品中氨基酸平均含量均高于滇重楼野生品。结论滇重楼栽培品与野生品中氨基酸种类和含量相似度较高,质量等同性较好,一定程度上证明栽培品可以替代野生品使用。
Objective A method of ultra-high performance liquid chromatography with pre-column derivatization was established for content determination of 15 amino acids in Paris polyphylla var.yunnanensis from different areas,and the amino acid contents in wild and cultivated Paris polyphylla var.yunnanensis were compared.Methods The contents of 15 kinds of amino acids in 27 batches of Paris polyphylla var.yunnanensis from 3 provinces were determined by using phenyl isothiocyanate(PITC)as derivative reagent after hydrolysis at 110℃for 22 h in 6 mol·L;hydrochloric acid solution.Statistical analysis of the contents of amino acids was carried out using t-test with SPSS20.0 software.Results The mass concentrations of 15 amino acids were all in the range of 0.354-30.912μg·mL^(-1),and the linear relationships were good(r=0.999 7-0.999 9),the average recoveries were between 95.12%and101.05%with RSDs were 1.32%-2.82%,and the detection limits were 0.031-0.089μg·mL^(-1),the limits of quantification were 0.104-0.297μg·mL^(-1).The contents of aspartic acid,glutamic acid and arginine were the highest in 27 samples of Paris polyphylla var.yunnanensis from 3 provinces.The average amino acid contents of cultivated products of Paris polyphylla var.yunnanensis was higher than those of wild products of Paris polyphylla var.yunnanensis.Conclusion The type and quality of amino acids in the cultivated and wild Paris polyphylla var.yunnanensis were highly similar,and the quality invariance of them was good.To a certain extent,this study proved that wild Paris polyphylla var.yunnanensis can be replaced by cultivated products.
作者
许凌峰
谷文超
李卓蔚
杜慧慧
郭冬琴
周浓
XU Lingfeng;GU Wenchao;LI Zhuowei;DU Huihui;GUO Dongqin;ZHOU Nong(College of Food and Biological Engineering,The Chongqing Engineering Laboratory for Green Cultivation and Deep Processing of the Three Gorges Reservoir Area’s Medicinal Herbs,Chongqing Three Gorges University,Chongqing 404120,China)
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2022年第2期235-241,共7页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
重庆市自然科学基金项目(cstc2018jcyjAX0267)
“成渝地区双城经济圈建设”科技创新项目(KJCX2020046)。
关键词
滇重楼
氨基酸
异硫氰酸苯酯
柱前衍生化
栽培品
野生品
超高效液相色谱
Paris polyphylla var.yunnanensis
amino acid
phenyl isothiocyanate
pre-column derivatization
cultivated plant
wild plant
ultra-high performance liquid chromatography(UPLC)
