摘要
为探究植物激素油菜素甾醇(BR)信号转导关键组分BES1对番茄(Solanum lycopersicum)叶片形态的调控作用,本研究构建了荧光标记微管的mBES1过表达BR超敏番茄植株,即mSlBES1-OX×SlTub6-eGFP杂交材料(B×T)。形态学和组织细胞学观察发现,mBES1过表达改变了叶片细胞的形态并产生卷叶表型。进一步蛋白质免疫印迹(Western Blot)试验结果表明,过表达mBES1番茄叶片微管蛋白表达量下降。同时,外源80μmol·L^(-1)微管解聚剂处理杂交材料,发现mBES1的过表达增加了细胞中微管对解聚剂的敏感性。综上,BR信号途径关键组分BES1主要通过调节微管蛋白量及其稳定性来影响番茄细胞的性状,并进一步调控叶片形态,这一结果为揭示BR通过微管调控番茄生长发育机理提供了重要的理论依据。
To explore tomato leaf shape regulation of BES1,a key component in brassinosteroids(BRs)signaling pathway,the experiment constructed the hybrid plant,of which mBES1 gene was overexpressed and the microtubules were labeled with the enhanced green fluorescent protein(eGFP)in the mBES1-OX background(mSlBES1-OX×SlTub6-eGFP,B×T).Morphological and cytological observation showed that BES1 overexpression resulted in curly leaf and leaf cells with altered shape.Western Blot assay confirmed that BES1 overexpression reduces the expression of tubulin in tomato leaves.In addition,microtubules were more sensitive to the microtubule-disrupting regent oryzalin in B×T than in SlTub6-eGFP.In conclusion,BES1 as a key component in BR signaling pathway affects the morphology of tomato cells and leaves by regulating the protein expression of tubulin and the stability of microtubules.These results provide important insight into how BR regulates the growth and development of tomato by mediating microtubules.
作者
梁冬怡
刘丽红
陈珊珊
苗慧莹
刘浩然
孟凡亮
邵志勇
汪俏梅
LIANG Dongyi;LIU Lihong;CHEN Shanshan;MIAO Huiying;LIU Haoran;MENG Fanliang;SHAO Zhiyong;WANG Qiaomei(Department of Horticulture,Zhejiang University/Key Laboratory of Horticultural Plant Growth,Development and Quality Improvement,Ministry of Agriculture and Rural Affairs,Hangzhou,Zhejiang 310058)
出处
《核农学报》
CAS
CSCD
北大核心
2022年第3期527-534,共8页
Journal of Nuclear Agricultural Sciences
基金
国家重点研发计划(2019YFD1001904-03)
国家基金重点项目(31830078)
国家青年基金项目(32002024)。
关键词
油菜素甾醇
BES1
微管
番茄
卷叶
brassinosteroids
BES1
microtubules
tomato
curly leaves
