摘要
以水稻平展叶品种Ketan Nangka和剑叶中度卷曲的广超丝苗杂交F1衍生的185个F2单株为定位群体,利用110个微卫星标记(SSR)对卷叶基因进行初步定位。在全基因组内构建分子遗传连锁图谱并进行QTL检测,在第4染色体长臂上定位到1个卷叶QTL(qRL-4-1)。它来自广超丝苗,与标记RM5473紧密连锁,加性效应为1.005,显性效应为-0.220,对表型的贡献率为7.27%,可能是一个新发现的QTL。同时在第5染色体长臂上定位到2个卷叶QTLs(qRL-5-9和qRL-5-10),两侧的标记分别为RM291-RM161和RM161-RM294,加性效应分别为1.812和1.347,显性效应分别为0.1196和0.1417,对表型的贡献率分别是16.08%和27.98%,其卷叶效应也来自广超丝苗,这2个位点验证了前人的研究结果。广超丝苗生育前期叶片并不表现卷曲,至生育后期才表现卷曲,其叶片卷曲基因在水稻株型育种上可能有较好的实用价值。
Two representative varieties, Guangchaosimiao, a semi-rolled flag leaf indica variety, and another one, Ketan Nangka, a flattend leafjavanica variety,were used to study roiled leaf trait. An F2 population with 185 plants, which was derived from F1 of Ketan Nangka x Guangchaosimiao, was used to QTL map for roiled leaf. Molecular genetic linkage map was constructed with 110 SSR markers, and QTL was detected throughout whole genome. One rolled leaf QTL （qRL- 4-1） which linked with molecular marker RM5473 on Chromosome 4, seemed to be a newly detected QTL. Its increasing leaf roiling allele came from the parent Guangchaosimiao. Its additive effect was 1. 005, dominant effect was - 0. 220, and could explain 7.27% of phenotypic variance. Meanwhile, two QTLs denoted as qRL-5-9 and qRL-5-10 on chromosome 5 were mapped between two flanking SSR markers, RM291-RM161 and RM161-RM294, with additive effects of 1. 812 and 1. 347, dominant effects of 0. 119 6 and 0. 141 7, and explanations for phenotypic variation of 16.08% and 27.98%, respectively. The QTLs might be useful since their rolled leaf effects were expressed in rice late growing stage rather than in early growing stage.
出处
《江苏农业学报》
CSCD
北大核心
2007年第1期5-10,共6页
Jiangsu Journal of Agricultural Sciences
基金
国家"863"计划项目(2003AA212040)
