摘要
目的探究3’-磷酸腺苷-5’-磷酸硫酸盐合酶2(PAPSS2)在胃癌组织和细胞中的表达情况及其对胃癌细胞侵袭和迁移的影响。方法利用GEPIA数据库分析PAPSS2在胃癌组织中的表达情况。收集陕西省人民医院2016年10月至2018年6月40例胃癌手术患者的癌组织及癌旁正常组织标本,进一步通过免疫组织化学染色进行验证。分析PAPSS2表达与胃癌患者临床病理资料和预后的关系。利用qPCR和免疫印迹法分别检测PAPSS2在胃癌细胞(HGC-27、SNU-1、AGS、SGC-7901)和正常胃黏膜细胞系(GES-1)的mRNA和蛋白水平表达差异。利用shRNA敲低胃癌细胞PAPSS2的表达,MTT检测其对细胞活性的影响,Transwell检测其对侵袭和迁移能力的影响。结果 (1)利用GEPIA数据库分析发现,PAPSS2在胃癌组织中高表达(P<0.05),且高表达PAPSS2的胃癌患者总体生存率(HR=1.5,P=0.017)和无病生存率(HR=1.6,P=0.014)显著降低。(2)PAPSS2在40例胃癌组织的免疫组织化学染色评分显著高于对应的癌旁组织(7.100±3.169 vs 3.425±2.263,P<0.001)。高表达PAPSS2的胃癌患者肿瘤浸润深度(P=0.015)和淋巴转移率更高(P=0.005)。(3)qPCR显示PAPSS2在胃癌细胞(AGS、HGC-27、SNU-1、SGC-7901)的mRNA表达水平明显高于GES-1细胞(F=15.45,P<0.001),免疫印迹法也得出了类似的结果。(4)MTT实验发现敲低PAPSS2对AGS和SGC-7901细胞的活性没有明显影响。(5)敲低PAPSS2后AGS细胞(386.9±73.75 vs 602.7±79.23,t=4.457,P=0.002)和SGC-7901细胞(237.8±77.60 vs 461.8±86.55,t=4.309,P=0.003)的迁移能力显著降低,AGS细胞(106.0±38.07 vs 201.3±48.79,t=3.446,P=0.009)和SGC-7901细胞(55.0±18.27 vs 93.6±21.07,t=3.093,P=0.015)的侵袭能力也明显降低。结论 PAPSS2在胃癌组织和细胞中呈高表达,其高表达促进了胃癌细胞的侵袭和转移,这可能是胃癌预后较差的重要原因之一。
Objective To investigate the expression of 3’-phosphoadenosine-5’-phosphosulfate synthase 2 (PAPSS2) in gastric cancer tissues and cells and its effects on the invasion and migration of gastric cancer cells.Methods The expression of PAPSS2 in gastric cancer were analyzed using the GEPIA database.The tissue specimens of 40 gastric cancer patients from October 2016 to June 2018 in Shaanxi Provincial People’s Hospital were collected for immunohistochemical staining.The association between PAPSS2 expression and clinicopathological data and prognosis of the gastric cancer patients was analyzed.The expression levels of PAPSS2 in gastric cancer cells (HGC-27,SNU-1,AGS and SGC-7901) and normal gastric mucosa cell line (GES-1) were tested by qPCR and Western blotting.The expression of PAPSS2 in gastric cancer cells was knocked down using shRNA,its effect on cell activity was detected using MTT,and its effect on invasion and migration ability was detected using Transwell method.Results (1) PAPSS2 was highly expressed in gastric cancer tissues according to GEPIA database (P<0.05).The overall survival rate (HR=1.5,P=0.017) and disease-free survival rate (HR=1.6,P=0.014) of patients with high PAPSS2 expression was significantly reduced.(2) Immunohistochemical staining revealed that the expression of PAPSS2 was significantly higher in gastric cancer tissues than in the corresponding paracancerous tissues (7.100±3.169 vs 3.425±2.263,P<0.001).Patients with high PAPSS2 expression had greater depth of invasion (P=0.015) and a higher rate of lymphatic metastasis (P=0.005).(3) qPCR results showed that the mRNA expression levels of PAPSS2 in gastric cancer cells (HGC-27,SNU-1,AGS and SGC-7901) were significantly higher than those in GES-1 cells.Similar results were obtained by Western blotting.(4) MTT assay showed that the knockdown of PAPSS2 had no significant effect on the activity of AGS and SGC-7901 cells.(5) The invasion and migration ability of AGS cells and SGC-7901 cells was significantly reduced after the PAPSS2 knockdown (P<0.05).Conclusion PAPSS2 is highly expressed in tissues and cells of gastric cancer,and its high expression promotes the invasion and metastasis of gastric cancer cells,which may be one of the important reasons for the poor prognosis of patients with gastric cancer.
作者
吴云桦
刘栋
薛飞
毛智军
高增战
普彦淞
Wu Yunhua;Liu Dong;Xue Fei;Mao Zhijun;Gao Zengzhan;Pu Yansong(The Second Department of General Surgery,Shaanxi Provincial People’s Hospital,Xi’an 710068,China)
出处
《中华普通外科学文献（电子版）》
CAS
2022年第1期27-31,共5页
Chinese Archives of General Surgery(Electronic Edition)
基金
陕西省教育厅重点科研计划项目(21JS041)
西安市科技计划项目(21YXYJ0091)
陕西省人民医院人才支持计划项目(2021JY-15)。
