摘要
目的:应用蛋白质组学技术辨析在血清样本中与运动性疲劳有关差异表达蛋白。方法:以17~21岁的10名成年男性为研究对象,建立运动疲劳模型。应用蛋白质组学技术,对运动疲劳前后血清中的蛋白质样品在相同条件下进行3次双向电泳,利用ImageMaster 2D platinum 5.0软件进行图像的强度校正、点检测、匹配等分析。根据蛋白质点表达量与所有匹配蛋白质点表达量总和的比值大于2.0,且同组3块胶图谱中都出现相同变化的蛋白点,被认为是差异蛋白质点。结果:研究发现,运动疲劳前后共检测出34个差异蛋白质点。最终鉴定出运动疲劳后表达上调的蛋白和表达下调的蛋白分别为6种,涉及到急性相蛋白、免疫相关蛋白、脂代谢相关蛋白和神经调节相关蛋白。结论:通过蛋白质组学技术高通量筛选后,发现与运动性疲劳有关差异表达蛋白有12种,其中,表达下调的α1-B-糖蛋白、丛生蛋白和结合珠蛋白,表达上调的α2-HS-糖蛋白、载脂蛋白E有必要进一步研究,确定其是否可以作为判断运动性疲劳的新的分子靶标。
Objective:The different protein expression related to exercise-induced fatigue in serum samples was discussed by using proteomics techniques.Method:In this study,10 male students aged 17 to 21 years were the research object building the model of sports.Protein samples in serum before and after exercise fatigue were screened applying the proteomics technology.The differential protein points were enzymolyzed being analyzed by the MALDI-TOF mass spectrometry and identified through searching the SwissProt Database.Result:A total of 34 different protein spots were detected before and after exercise fatigue.Finally,12 proteins were identified as up-regulated and down-regulated after exercise fatigue,which involves acute phase proteins,immune-related proteins,lipid metabolism-related proteins and neuroregulatory proteins.Conclusion:After high throughput screening by proteomics technique,12 differentially expressed proteins related to exercise-induced fatigue were found,among which,the down-regulatedα1-B-glycoprotein,fascicular protein and binding globin,and the up-regulatedα2-hs-glycoprotein and apolipoprotein E should be further studied to determine whether they can be used as a new molecular target for the determination of exercise-induced fatigue.
作者
张婧
翟琳
万炳军
周园
李小龙
ZHANG Jing;ZHAI Lin;WAN Bingjun;ZHOU Yuan;LI Xiaolong(School of Physical Education,Shaanxi Normal University,Xi'an 710119,Shaanxi,China)
出处
《山东体育科技》
2021年第6期50-56,共7页
Shandong Sports Science & Technology
基金
陕西省科技厅区域创新引导计划项目“运动+膳食”健康管理智能云平台的研制与应用(编号:2020QFY01-03)。
关键词
运动性疲劳
蛋白质组
血清蛋白
exercise-induced fatigue
proteomics
serum protein
