摘要
目的研究冻干保存方式以及激活时间对富血小板血浆(platelet rich plasma,PRP)生长因子含量的影响。方法用来自20位健康献血者的血液制备PRP,血小板浓度为(1031±150.1)×10^(9)/L。根据不同保存方式将每份PRP样本分为5组,即新鲜PRP组、冷冻PRP激活组、激活后PRP冷冻组、冻干PRP激活组和激活后PRP冻干组。检测每组PRP中TGF-β1、VEGF、PDGF-BB含量。结果冻干PRP激活组的TGF-β1和PDGF-BB含量与新鲜PRP组相比差异无统计学意义,而VEGF含量较新鲜PRP组有所升高且差异有统计学意义(P<0.05)。其他3组的TGF-β1和VEGF含量与新鲜PRP组相比差异无统计学意义,而PDGF-BB的差异具有统计学意义。结论相比较新鲜PRP组,冷冻和冻干保存PRP不影响其三种主要生长因子的释放,而相较冷冻PRP而言,冻干保存对储存条件要求更低,更利于拓展PRP的使用范围。
Objective To study the effect of freeze-drying preservation method and activation time on platelet rich plasma(PRP)growth factor(GFs)content.Methods 20 PRPs were collected from healthy blood donors,of which platelet concentration was(1031±150.1)×10^(9)/L.Each PRP sample is divided into 5 groups,(1)fresh PRP(PRP),(2)frozen PRP activated by calcium gluconate(F-Ca PRP),(3)PRP activated by calcium gluconate followed by freezing(Ca-F PRP),(4)PRP freeze-dried first,then activated by calcium gluconate(FD-Ca PRP),(5)PRP activated by calcium gluconate followed by freeze drying(Ca-FD PRP).The concents of transforming growth factor-β1(TGF-β1),vascular endothelial growth factor(VEGF),and platelet-derived growth factor-BB(PDGF-BB)were quantified by ELISA.Results TGF-β1 and PDGF-BB concentrations in FD-Ca PRP were not statistically different from PRP,while the VEGF was elevated and statistically different compared with the PRP.TGF-β1 and VEGF concentrations in the other three groups were not statistically different from PRP,while the differences in PDGF-BB were statistically significant.Conclusion The freezing and freeze-drying on PRP did not affect the concentrations of the three main growth factors from PRP,and compared with freezing on PRP,freeze-drying was less required with storage conditions.It suggested that wider possibilities of freeze-dried PRP for clinical applications.
作者
罗开云
王淑君
毛平平
孙杨子
齐清
栾建凤
LUO Kai-yun;WANG Shu-jun;MAO Ping-ping(Department of Blood Transfusion Medicine,Jinling Hospital Nanjing University,School of Medicine,Nanjing 210002)
出处
《临床输血与检验》
CAS
2022年第1期70-73,共4页
Journal of Clinical Transfusion and Laboratory Medicine
基金
江苏省卫健委面上项目(No.H2019110)
东部战区总医院院内课题(No.YYMS2021019)
全军重大课题(No.ANJ13J001)资助。
关键词
富血小板血浆
冷冻
冻干
生长因子
Platelet-rich plasma
Frozen
Freeze-dried
Growth factor
