摘要
To explore interleukin-6(IL-6)production and characterize lipid accumulation in L02 hepatocytes induced by sodium oleate.L02 hepatocytes were incubated with 0,37.5,75,150,300,600,or 1,200μmol/L sodium oleate for 24 h,and the supernatant was collected to detect the concentration of IL-6.L02 hepatocytes were incubated with 300,150,75,or 0μmol/L sodium oleate for 0–24 h.The supernatant was collected for detection of IL-6 and free fatty acids.L02 hepatocytes treated with 300μmol/L sodium oleate for 0–24 h were stained with Oil Red O.With extended sodium oleate incubation time,IL-6 levels increased,and free fatty acids decreased.After 24 h incubation,IL-6 levels increased as sodium oleate increased from 37.5 to 300μmol/L(P<0.05 for 37.5μmol/L,P<0.01 for 75μmol/L and P<0.001 for concentrations 150μmol/L or higher).Lipid accumulation increased as the sodium oleate concentration and incubation time increased.Oil Red O staining intensified with incubation time extending beyond 2 h.IL-6 production and lipid accumulation in L02 hepatocytes are influenced by sodium oleate in a dose-and time-dependent manner.
基金
Toxicity Evaluation of Key Contaminants in Health Food by Cell-based Test Models and the Mechanism Analysis[2018YFC1602104]。
