摘要
Objective:Autophagy is a programmed cell death procedure,which has essential functions in tumorigenesis.However,its temporal expression and function under different status are yet to be determined.This study aims to investigate the temporal expression of autophagy and its possible function in 7,12-dimethylbenz[a]anthracene(DMBA)-induced hamster buccal-pouch cancer model(HBPCM).Methods:A total of 50 hamster buccal-pouch tumorigenesis models were established by painting DMBA for 4,8,10 and 13 weeks.The expression and subcellular localization of LC3,Beclin 1 and Bcl-2 in buccal lesions were evaluated by immunohistochemical staining and Western blotting.DNA damage was observed by immunohistochemical staining of 8-oHdG.The relationship between Beclin 1 and Bcl-2 was analyzed by immunofluorescence colocalization.Results:The expression levels of LC3 and Beclin 1 associated with autophagy in the experimental buccal pouch of HBPCM were significantly upregulated after 4 weeks(P<0.05),but gradually downregulated after 13 weeks of HBPCM induction.By contrast,the expression level of Bcl-2 was significantly upregulated after 13 weeks.The co-localized regions of Bcl-2 and Beclin 1 peaked after 4 weeks and then decreased gradually.The DNA damage in epithelial cells increased slightly after 4 weeks,and then rapidly decreased over the next 2 months.Conclusion:Autophagy is motivated by a tumor suppressor that diminishes carcinogen-induced DNA damage.However,autophagy is gradually suppressed,which may be attributed to the interaction between Bcl-2 and Beclin 1.This result indicates that the promotion of autophagy may suppress malignant transformation and provide new insights on future potential treatments of HBPCM.
