摘要
为探究邯郸地区空气中直径≤2.5μm的细颗粒物(diameter≤2.5μm particular matter,PM2.5)对人外周血T细胞转化功能的影响,并从细胞氧化应激水平变化角度探讨其可能机制,以植物凝集素(phytohemagglutin,PHA)诱导健康人外周血T细胞转化,同时加入不同剂量PM2.5共孵育,检测T细胞增殖抑制率、转化值及细胞培养上清液中IL-6、TNF-α、8-OHdG和细胞内ROS水平变化。结果显示,染毒时间相同时,PM2.5对T细胞增殖抑制率的影响随染毒剂量的增加显著上调(均P<0.05),而转化值随染毒剂量的增加显著降低(均P<0.05);当PM2.5剂量相同时,其对T细胞的增殖抑制率随染毒时间的延长显著上升(均P<0.05)。PM2.5染毒时间相同时,300μg/mL组细胞上清液中IL-6、8-OHdG水平显著高于100μg/mL组(均P<0.05),且TNF-α和ROS水平显著高于30μg/mL组(均P<0.05),100μg/mL组细胞上清液中IL-6、8-OHdG和ROS水平显著高于30μg/mL组(均P<0.05)。由此,PM2.5可能通过刺激细胞产生过多的活性氧自由基导致DNA氧化损伤,从而抑制PHA诱导的人外周血T细胞转化并促进炎性因子的产生。
To study the effect of fine particular matter less than 2.5μm in diameter(PM2.5)from Handan region on the transformation of human peripheral blood T cells,and to explore the mechanism at the cell oxidative stress level,T cell transformation was induced by phytohemagglutin(PHA)and co-incubated with different doses of PM2.5.The T cell proliferation inhibition rate,transformation value and the levels of IL-6,TNF-αand 8-OHdG in cell culture supernatant and intracellular reactive oxygen species(ROS)level were measured.The results showed that when the exposure time was constant,the PM2.5-induced T cell proliferation inhibition rate was significantly increased with the increase of PM2.5 dose(all P<0.05),while the transformation value was at the same time significantly reduced(all P<0.05).When PM2.5 dosage was constant,the T cell proliferation inhibition rate was significantly increased with the exposure prolongation(all P<0.05).The levels of IL-6 and 8-OHdG in the supernatant of 300μg/mL PM2.5 group were significantly higher than those in the 100μg/mL group(all P<0.05),and the TNF-αand ROS levels in the supernatant of 300μg/mL group were significantly higher than those in the 30μg/mL group(all P<0.05).The levels of IL-6,8-OHdG and ROS were higher in the 100μg/mL group than those in the 30μg/mL group(all P<0.05).As a result,PM2.5 can inhibit the transformation of human peripheral blood T cells induced by PHA via stimulating cells to produce excessive reactive oxygen radicals which leads to DNA oxidative damage and further inhibits T cell transformation induced by PHA and increases the production of inflammatory factors.
作者
李卫霞
刘晓霞
陈剑华
郭旭丽
王雪玲
霍忠超
LI Wei-xia;LIU Xiao-xia;CHEN Jian-hua;GUO Xu-li;WANG Xue-ling;HUO Zhong-chao(Department of Immunology,Medical College of Hebei University of Engineering,Handan 056038,China;Department of Oncology,Affiliated Hospital of Hebei University of Engineering,Handan 056038,China)
出处
《现代免疫学》
CAS
北大核心
2021年第5期397-401,共5页
Current Immunology
基金
邯郸市科学技术研究与发展计划项目(1623208260-6)。
关键词
细颗粒物
淋巴细胞
自由基
氧化损伤
转化
fine particular matter
lymphocyte
free radical
oxidative damage
transformation
