摘要
【背景】细菌耐药已成为全球严重的公共卫生问题,养殖业是细菌耐药性产生的重要源头之一。我国正在全力推进"减抗、限抗、禁抗"战略。新型安全高效的抗生素替代品成为当前养殖业的重要需求。噬菌体因其能有效裂解细菌被认为是一个重要的突破口,但噬菌体作为活体微生物,在保存和使用时存在稳定性差、利用率低等问题。【目的】制备噬菌体粉剂,提高噬菌体的抗逆性和稳定性,为噬菌体在养殖业中的应用提供技术支持。【方法】选用大肠杆菌噬菌体BpEP4,采用嵌段式聚醚F-68包被噬菌体,然后负载于脱脂米糠制得噬菌体粉剂,双层琼脂平板法测定粉剂的噬菌体效价,研究其耐高温性能、pH稳定性与常温保存稳定性。【结果】噬菌体粉剂可以在100℃保持噬菌体的活性,pH耐受范围为2.0-12.0。常温下保存3个月噬菌体效价无明显降低。【结论】粉剂显著提高了噬菌体的抗逆性与稳定性,具有很好的应用价值和推广前景。
[Background]Antimicrobial resistance is a serious threat to global public health,and the animal breeding is an important source of pathogen spreading.Further,our country has created a set of strategies to combat rising resistance,which include of optimizing the use(and preventing the overuse)of antibiotics.Since,novel,safe and efficient alternatives to antibiotic are highly in demand.Bacteriophages(phages)are being considered as promising potential antibacterial agents.However,phages,as living microorganisms,have some drawbacks such as poor stability and low utilization during storage and use.[Objective]Phage powder was prepared to improve the stress resistance and stability of phage,and to provide technical support for the application of phage in the breeding industry.[Methods]In this study,we produce a phage powder of E.coli phage BpEP4 with block polyether F-68 and the defatted rice bran excipents.The double agar plate method was used to study its stability against high temperature,pH stability and storage stability at room temperature.[Results]The phage powder can maintain the activity of phage at 100℃and could survive in a pH range of 2.0-12.0.There is no significant reduction in phage titer after 3 months at room temperature.[Conclusion]The phage powder can significantly improve the stress resistance and stability of the phage,and has good application value and promotion prospects.
作者
王合叶
徐沁妍
姜越
张莉莉
孙利厂
王冉
WANG Heye;XU Qinyan;JIANG Yue;ZHANG Lili;SUN Lichang;WANG Ran(Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base,Ministry of Science and Technology,Institute of Food Safety and Nutrition,Jiangsu Academy of Agricultural Sciences,Nanjing,Jiangsu 210014,China;School of Food Science,Nanjing Xiaozhuang University,Nanjing,Jiangsu 211171,China;School of Food and Biological Engineering,Jiangsu University,Zhenjiang,Jiangsu 212013,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2021年第9期3109-3115,共7页
Microbiology China
基金
国家重点研发计划(2021YFE0101800)
江苏省农业科技自主创新资金(CX(19)2014)。
关键词
噬菌体
噬菌体粉剂
抗逆性
稳定性
bacteriophage
bacteriophage powder
stress resistance
stability
