摘要
从上海国家森林公园土壤样本中筛选到1株菌株,经过生理生化鉴定和16S rDNA序列分析,该菌属肠杆菌属并命名为Enterobacter sp.MF024。Enterobacter sp.MF024全基因组测序结果表明该菌株包含从头合成途径和艾氏途径合成2-苯乙醇所有关键酶的编码基因。分别以葡萄糖、L-苯丙氨酸为底物进行生物转化实验,并以苯乙醛、苯丙酮酸等合成途径中间产物为底物进行验证,气相色谱-质谱法、红外光谱分析结果进一步说明Enterobacter sp.MF024具备两种2-苯乙醇合成途径,且该菌利用从头合成途径和艾氏途径产2-苯乙醇产量分别达到0.56、1.15 g/L。该菌株以葡萄糖为碳源生物合成2-苯乙醇极具应用前景。
A bacterial strain was isolated from soil samples collected from the Gulf Forest Park in Shanghai.Biochemical identification and 16S rDNA sequence analysis indicated that the strain belonged to the genus Enterobacter and was designated as Enterobacter sp.MF024.Whole-genome sequence analysis showed that this strain had all key enzyme encoding genes involved in the Ehrlich pathway and de novo synthesis pathway for 2-phenylethanol.Then,biotransformation experiments were carried out using glucose as the carbon source and L-phenylalanine as the substrate.The key intermediates in the synthetic pathways such as phenylacetaldehyde and phenylpyruvate were further used as the substrates for validation experiments.Enterobacter sp.MF024 possesses both 2-phenylethanol synthesis pathways according to the results from gas chromatography-mass spectrometry and infrared spectrometry.The 2-phenylethanol yield was 0.56 g/L in the de novo synthesis pathway and 1.15 g/L in the Ehrlich pathway.This strain has great potential in the production of 2-phenylethanol by using glucose as the carbon source.
作者
荣绍丰
伍进
管世敏
蔡宝国
张硕
李茜茜
RONG Shaofeng;WU Jin;GUAN Shimin;CAI Baoguo;ZHANG Shuo;LI Qianqian(School of Perfume and Aroma Technology,Shanghai Institute of Technology,Shanghai 201418,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2021年第16期69-75,共7页
Food Science
基金
国家自然科学基金面上项目(31972165)。
关键词
肠杆菌
2-苯乙醇
艾氏途径
从头合成途径
Enterobacter
2-phenylethanol
Ehrlich pathway
de novo synthesis pathway
