摘要
为了探究外源NO供体硝普钠(sodium nitroprusside,SNP)对盐胁迫下桔梗幼苗生长、生理生化指标及总皂苷积累的影响,以一年生桔梗幼苗为试验材料,采用不同浓度SNP(30,90,150μmol/L)喷施400 mmol/L NaCl胁迫处理下的桔梗幼苗,分别在处理10、20和30 d后测定桔梗幼苗的生物量、丙二醛(malondialdehyde,MDA)含量、抗氧化酶类超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)、过氧化氢酶(catalase,CAT)、渗透调节物质脯氨酸、可溶性糖含量等生理生化指标,测定胁迫30 d后桔梗根中桔梗总皂苷含量。结果表明,400 mmol/L NaCl胁迫后显著抑制了桔梗幼苗的生长和生理生化指标,喷施不同浓度SNP处理后,不同程度地降低了MDA含量,在胁迫20 d时,90μmol/L的SNP(中浓度SNP)处理后,与单盐胁迫组相比,根与叶中与分别降低了11.2%和42.1%。抗氧化酶类(SOD,POD,CAT)活性整体呈现先升高后降低的趋势,喷施SNP后活性增强,以胁迫20 d时,中浓度SNP处理后,效果更为显著。渗透调节物质脯氨酸和可溶性糖含量随胁迫时间的延长持续增加,中浓度SNP处理下含量达到最大。高浓度SNP处理促进了桔梗根中总皂苷的积累,比单盐胁迫组增加了88.9%,低浓度和中浓度无显著促进作用。本研究为提高桔梗幼苗的耐盐性,提高其产量和品质提供一种新的手段,为今后桔梗的规范化栽培提供理论依据。
In order to investigate the effects of exogenous NO donor sodium nitroprusside(SNP)on the growth,physiological and biochemical characteristics and theaccumulation of total saponins in Platycodon grandiflorus(Jacq.)A.DC.seedlings under salt stress,the one year old P.grandiflorus(Jacq.)A.DC.seedlings was used as the experimental material,and SNP with different concentrations(30,90,150μmol/L)was used to spray the seedlings under 400 mmol/L NaCl stress.The physiological and biochemical indexes such as biomass,malondialdehyde(MDA)content,superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),osmotic substances proline and soluble sugar contentof P.grandiflorus(Jacq.)A.DC.seedlings were measured after 10,20,30 d treatment.The total saponins content in roots were measured after 30 d treatment.The results showed that 400 mmol/L NaCl stress significantly inhibited the growth and physiological and biochemical indexes of P.grandiflorus(Jacq.)A.DC.seedlings.Spraying different concentrations of SNP could decrease the MDA content.Compared with the salt stress group,the roots and leaves decreased by 11.2%and 42.1%respectively after treatment with 90μmol/L SNP(Middle concentration SNP)on 20 d.The activities of antioxidant enzymes(SOD,POD,CAT)increased firstly then decreased.After spraying SNP,the activity increased.When the stress was applied for 20 d.The effect of middle concentration SNP is more significant on 20 d.The content of proline and soluble sugar continued to increase with the extension of stress time,and reached the maximum under the treatment of middle concentration SNP.Highconcentration SNP treatment promoted the accumulation of total saponins in the roots of P.grandiflorus(Jacq.)A.DC.,which increased by 88.9%compared with thesalt stress group.Low and middle concentrations had no significant promoting effect.This study provides a new method for improving the salt tolerance of P.grandiflorus(Jacq.)A.DC.seedlings,improving their yield and quality,and providing a theoretical basis for the standardized cultivation of P.grandiflorus(Jacq.)A.DC.in the future.
作者
贾鑫
王晓琴
张英
于娟
岳鑫
薛焱
丛迎楠
Jia Xin;Wang Xiaoqin;Zhang Ying;Yu Juan;Yue Xin;Xue Yan;Cong Yingnan(School of Pharmacy,Inner Mongolia Medical University,Hohhot,010110)
出处
《分子植物育种》
CAS
北大核心
2021年第10期3432-3440,共9页
Molecular Plant Breeding
基金
国家自然科学基金(81960687)
内蒙古医科大学博士启动基金(YKD2018BSJJ)
内蒙古医科大学“青年领创”团队联盟项目(QNLC-2020048)共同资助。
