摘要
目的在生物方剂分析药理(bioanalyticalpharmacology,BAP)策略的指导下,检测柴胡疏肝散水提物中具有抗抑郁作用的可吸收生物活性物质,为柴胡疏肝散抗抑郁疗效的质量标志物(quality marker,Q-Marker)评价提供依据。方法运用液相色谱-串联质谱(LC-MS/MS)测定柴胡疏肝散水提取物中的28种成分;大鼠ig柴胡疏肝散水提取物,定性分析大鼠血浆中的化合物,筛选生物活性物质,探索具有抗抑郁作用的Q-Marker。结果从柴胡疏肝散水提物中的28种成分中确定了吸收入血的22个成分,筛选出的10个生物活性物质具有与柴胡疏肝散类似的抗抑郁疗效。结论芍药内酯苷、阿魏酸、柚皮苷、橙皮苷、水合橙皮内酯、甘草酸、柴胡皂苷A、川陈皮素、橘皮素、甘草次酸为柴胡疏肝散抗抑郁作用的Q-Marker。
Objective To detect the antidepressant absorption active compounds in water extract of Chaihu Shugan Powder,and provide a basis for evaluation of quality marker(Q-Marker)of Chaihu Shugan Powder antidepressant effect under the guidance of bioethnopharmaceutical analytical pharmacology(BAP)strategy.Methods LC-MS/MS was used to determine the 28 compounds in water extract of Chaihu Shugan Powder.Rats were ig given Chaihu Shugan Powder,compounds in rat plasma were analyzed and biological activity substances were screened to explore Q-Marker with antidepressant effects.Results Among 28 components in water extract of Chaihu Shugan Powder,22 components were determined to be absorbed into blood.A total of 10 biological activity substances had antidepressant effects similar to Chaihu Shugan Powder.Conclusion Paeoniflorin,ferulic acid,naringin,hesperidin,hydrated hesperidin,glycyrrhizic acid,saikosaponin A,lichenpin,hesperetin,and glycyrrhetinic acid are Q-Markers for antidepressant effect of Chaihu Shugan Powder.
作者
吴磊
刘雅琳
黄熙
WU Lei;LIU Ya-lin;HUANG Xi(Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China;Nanjing University of Chinese Medicine,Nanjing 210029,China;Henan University of Chinese Medicine,Zhengzhou 450046,China)
出处
《中草药》
CAS
CSCD
北大核心
2021年第9期2617-2625,共9页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(81573797)。
关键词
柴胡疏肝散
质量标志物
生物方剂分析药理策略
抑郁症
生物活性物质
Chaihu Shugan Powder
quality marker
bioanalytical pharmacology strategy
depression
biologically active substance
