摘要
从极端污染的土壤中 ,分离到一株对草苷膦有极端抗性的细菌菌株HTG7。它能够在以草苷膦为选择压力的限制性培养基上生长 ,其最高耐受能力为 5 0 0mmol/L。采用PCR扩增获得该菌 16SrDNA片段。核苷酸序列分析表明 ,该菌的 16SrDNA核苷酸序列与盐单胞菌属同源性最高 ,在 95 %～ 98%之间。在细菌系统分类学上 ,HTG7归属盐单胞菌属Variabil。
A bacterial strain HTG7,which can grow in concentrations of glyphosate 500mmol/L in the M9 broth, was isolated from extremely glyphosate-polluted soil. The sequence analysis of a 1497 bp 16S rDNA fragment from total DNA of HTG7 strain by PCR showed that HTG7 was high 16S rDNA sequence homelogy with Halomonas bacteria. And the similarity was between 95% and 98%. In phylogenetic framework of baterial classification, HTG7 belongs to Halomonas Variabil.
出处
《高技术通讯》
EI
CAS
CSCD
2002年第12期25-28,共4页
Chinese High Technology Letters
基金
86 3计划 ( 2 0 0 1AA2 14 2 31)
国家转基因植物研究与产业化专项课题 (J0 0 - A- 0 10 )资助项目。
