摘要
氢氧化铝作为疫苗佐剂被广泛运用的重要原因是能够被抗原提呈细胞(antigen-presenting cells,APC)如树突状细胞(dendritic cells,DC)、巨噬细胞(macrophages,M?)识别、吞噬,并增强其提呈抗原的能力,实现有效免疫应答.APC吞噬受铝佐剂辅佐的抗原后将激活溶酶体、线粒体等细胞器,其中线粒体表现尤为明显:如线粒体活性氧(mitochondrial reactive oxygen species,m ROS)、线粒体膜电位(mitochondrial membrane potential,ΔΨm)及线粒体糖脂代谢等会发生一系列变化.我们也发现了巨噬细胞可以快速吞噬铝佐剂辅佐的抗原,并快速激活机体免疫应答.那么,发生"快吞噬、快激活"的物质基础应该需要更多的能量支撑,为此我们推测线粒体的生物学行为会更加活跃,在实现免疫应答后也许对M?造成"大伤害".本文将对该现象做一综述并引出关于下一步需要解决的问题的思考.
The important reason why aluminum hydroxide is widely used as a vaccine adjuvant is that it can be recognized and phagocytosed by antigen presenting cells(APC)such as dendritic cells(DC)and macrophages(M?).It can also enhance the immune response effectively by affecting the presentation ability of antigens.APC phagocytosis of antigens adjuvanted with aluminum adjuvant could activate lysosomes,mitochondria and other organelles,especially the mitochondria.A series of changes would occur in the mitochondria,including changes among reactive oxygen species(ROS),mitochondrial membrane potential(ΔΨm)and mitochondrial glycolipid metabolism,etc.Our research found that the antigens containing aluminum adjuvants can be rapidly phagocytized by macrophages and then an effective immune response can be induced.It is easy to find that the material basis of"fast phagocytosis and fast activation"should require more energy supporting.For this reason,we speculate that the biological behavior of mitochondria will be more active,and may cause"huge damage"to M?after the immune response is achieved.This review will give an overview of this phenomenon and elicit the thinking that needs to be solved in the next step.
作者
黄丽婷
梁桂兆
吕凤林
HUANG Li-Ting;LIANG Gui-Zhao;Lü Feng-Lin(College of Bioengineering,Chongqing University,Chongqing 400030,China;Key Laboratory of Biorheological Science and Technology,Chongqing 400030,China)
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2021年第5期560-569,共10页
Progress In Biochemistry and Biophysics
关键词
铝佐剂
巨噬细胞
线粒体
ATP
纳米颗粒
aluminum adjuvants
macrophage
mitochondria
ATP
nanoparticles
