摘要
目的:建立外周血中人乳腺珠蛋白(hMAM)荧光免疫层析快速定量检测方法。方法:采用双抗体夹心法制备免疫层析试纸条,并评价线性范围、精密性、特异性等性能指标。结果:实现了外周血中hMAM定量检测,线性范围0.312~20 ng/ml,批内变异系数均小于10%,批间变异系数均小于15%,平均回收率102.6%,与癌胚抗原(CEA)、癌抗原(CA153)无明显交叉反应,ELISA平行检测46例临床血清相关性良好。结论:建立了定量检测hMAM的时间分辨荧光免疫层析方法,为乳腺癌诊断和预后提供一种辅助检测手段。
Objective:To establish a fluorescence immunochromatographic method for rapid and quantitative detection of human mammaglobin(hMAM)in peripheral blood.Methods:The double antibody sandwich method was used to prepare immunochromatographic test strips,and the performance indicators such as linear range,precision and specificity were evaluated.Results:The quantitative detection of hMAM in peripheral blood was realized.The linear range was 0.312~20 ng/ml.The intra-assay coefficient of variation was less than 10%,the inter-assay coefficient of variation was less than 15%,and the average recovery was 102.6%.There was no obvious cross-reaction with carcinoembryonic antigen(CEA)and cancer antigen(CA153).There was a good correlation with46 clinical serum detected in parallel with ELISA kit.Conclusion:A time-resolved fluorescence immunochromatographic method for quantitative detection of hMAM has been established,which provides an auxiliary detection method for the diagnosis and prognosis of breast cancer.
作者
崔云会
陈楠
王云龙(指导)
明亮
李玉林
王继创
CUI Yun-Hui;CHEN Nan;WANG Yun-Long;MING Liang;LI Yu-Lin;WANG Ji-Chuang(Laboratory Department of the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2021年第8期975-978,共4页
Chinese Journal of Immunology
基金
河南省科技攻关项目(192102310418)。
关键词
人乳腺珠蛋白
荧光免疫层析法
乳腺癌
Human mammaglobin
Fluorescence immunochromatography
Breast cancer
