摘要
为解决荧光免疫分析灵敏度低,线性范围窄等问题,设计一款基于免疫层析技术和时间分辨荧光微球的免疫层析分析仪。该系统以荧光层析试剂卡为检测对象,标记物为时间分辨荧光微球。仪器的激发光源为单色LED(340 nm),通过光电二极管检测试剂卡T线和C线上荧光微球发出的荧光,结合标准浓度曲线即可计算出待测物的浓度。经实验测试,仪器在0.5~200 mg/mL范围内具有良好的线性,线性相关系数为R^(2)=0.9988;仪器精密度良好,台内CV小于1%,台间CV小于2.5%。仪器操作简单,检测快速,可广泛应用于POCT、毒品检测、食品安全检测等领域。
In order to solve the problems of low sensitivity and narrow linear range of fluorescence immunoassay,an immune analyzer based on immunochromatography assay and time-resolved fluorescence microparticles was designed.In the system,the detection object is the fluorescent reagent card,the marker of which is the time-resolved fluorescence microparticles.The excitation light is monochromatic LED(340 nm).The fluorescence emitted by the fluorescence microparticles on the T line and C line of the reagent card is detected by photodiode.The concentration of the measured substance can be calculated using the fluorescence value together with the standard concentration curve.The results show that the immune analyzer shows up good linearity in the range of 0.5~200 mg/mL,with linear correlation coefficient R^(2)=0.9988.The precision of the immune analyzer is also pretty good since the instrument CV is generally less than 1%,and the inter-instrument CV is no more than 2.5%.The merit of simple operation and fast detection allows the immune analyzer to be applied in a wide range of fields,such as the POCT,drug detection,food safety detection and so on.
作者
刘艳萍
杨光光
何海英
陈锐彬
Liu Yanping;Yang Guangguang;He Haiying;Chen Ruibin(Guangdong Polytechnic of Industry and Commerce,Guangzhou 510510,China;Foshan University,Foshan 528000,China)
出处
《电子测量技术》
北大核心
2021年第7期133-138,共6页
Electronic Measurement Technology
基金
广东省青年创新人才项目(自然科学)(2018GkQNCX055)资助
关键词
时间分辨荧光微球
免疫层析分析仪
光学检测
曲线拟合
time-resolved fluorescence microparticles
immunochromatography analyzer
optical detection
curve fitting
