摘要
为探究盐胁迫下丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)对桑树生长状况和光合作用生理生化特征的影响,以桑树品种桂桑优12为材料进行盆栽试验,设置不接种对照和接种根内根孢囊霉(Rhizophagus intraradices)2个处理,接种45 d后再用不同浓度(0、100、200 mmol/L)NaCl溶液进行胁迫处理。1个月后的调查结果表明,盐胁迫下AMF对桑树形成较高的侵染率,桑树对AMF有较大的依存度。在NaCl溶液浓度为100 mmol/L时,接种AMF能够增加桑树的株高以及叶、茎、根的鲜质量和干质量,其中地上部分鲜质量增加最为明显,叶和茎鲜质量比未接种对照分别提高117.24%、102.56%;接种AMF桑树的根表面积、根平均直径、根体积、总根长和根系活力分别提高42.96%、16.21%、44.75%、46.70%和97.35%;叶片中的叶绿素a和b含量分别提高57.09%、54.48%,净光合速率(Pn)、气孔导度(Gs)和蒸腾速率(Tr)分别提高50.54%、47.28%和46.78%,与未接种对照之间存在显著差异。在NaCl溶液浓度为100、200 mmol/L时,接种AMF后桑树叶片的丙二醛(MDA)含量分别降低36.82%、31.80%,超氧化物歧化酶(SOD)活性分别提高15.86%、30.33%,可溶性蛋白含量分别提高35.12%、42.13%,有效缓解了盐胁迫对桑树叶片造成的生理损伤。综上所述,接种AMF能够促进桑树地上部及根系的生长,提高根系活力,增加叶绿素含量,提高光合作用效率,增强活性氧清除能力,降低质膜过氧化程度,提高渗透调节能力,从而增强桑树的耐盐性。
In order to explore the effects of arbuscular mycorrhizal fungi(AMF)on the growth and photosynthetic physiological and biochemical characteristics of mulberry tree under salt stress,a pot experiment was carried out taking mulberry variety Guisangyou 12 as the experimental material,and two treatment groups including no inoculation and inoculation of Rhizophagus intraradices were first treated for 45 d,and then treated with different concentrations of NaCl solution(0,100,200 mmol/L).Investigation results after one month indicated that AMF had a higher infection rate on mulberry tree under salt stress,and mulberry tree had greater dependence on AMF.When the NaCl concentration is 100 mmol/L,inoculation with AMF can increase the plant height,and fresh weight and dry weight of leaves,stems and roots.Among them,fresh weight of the aboveground part increased the most,and fresh weight of leaves and stems increased by 117.24%and 102.56%,respectively.Besides,root surface area,average root diameter,root volume,total root length and root vitality of mulberry tree inoculated with AMF increased by 42.96%,16.21%,44.75%,46.70%and 97.35%,respectively.Meanwhile,contents of chlorophyll a and b in leaf each increased by 57.09%and 54.48%,net photosynthetic rate(Pn),stomatal conductance(Gs)and transpiration rate(Tr)increased by 50.54%,47.28%and 46.78%,respectively,all of which were significantly different from those of the no inoculation treatment group.When NaCl concentrations were 100 and 200 mmol/L,malondialdehyde(MDA)contents in leaf of mulberry after inoculation with AMF decreased by 36.82%and 31.80%,activity of superoxide dismutase(SOD)increased by 15.86%and 30.33%respectively,the soluble protein contents increased by 35.12%and 42.13%respectively,which effectively alleviated the physiological damage on mulberry leaves caused by salt stress.In summary,inoculation with AMF can promote the growth of mulberry shoots and roots,increase root vitality,increase chlorophyll content,increase photosynthesis efficiency,enhance active oxygen scavenging ability,reduce plasma membrane peroxidation,and improve osmotic adjustment ability,thereby enhancing the salt tolerance of mulberry.
作者
郑亚茹
唐明
Zheng Yaru;Tang Ming(College of Forestry,Northwest A&F University,Yangling Shaanxi 712100,China;State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,Guangdong Provincial Key Laboratory of Forest Plant Germplasm Innovation and Utilization,College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou 510642,China)
出处
《蚕业科学》
CAS
CSCD
北大核心
2020年第6期669-677,共9页
ACTA SERICOLOGICA SINICA
基金
国家自然科学基金项目(32071639)。
关键词
丛枝菌根真菌
桑树
盐胁迫
光合作用
Arbuscular mycorrhizal fungi
Mulberry
Salt stress
Photosynthesis
