摘要
石漠化地区桑树根际AM真菌具有多样性,鉴定了3属(球囊霉属、无梗囊霉属和巨孢囊霉属)16种AM真菌,其中球囊霉属和无梗囊霉属为石漠化地区AM真菌优势属.以AM真菌和桑苗为试材,网室试验池中分别对生长2个月的无菌根桑苗接种摩西球囊霉、根内球囊霉和细凹无根囊霉3个AM真菌菌种,45d后测量接种和不接种桑苗的菌根侵染率、菌根依赖性、生长量和光合特性等指标.结果表明:网室中接种45 d的桑苗菌根侵染率最高达到(38.67±1.44)%,桑苗对真菌菌根依赖性最高达到(203.03±14.93)%,接种菌根桑苗株高、茎粗、叶片数、第五片叶大小、主根长、植株鲜质量、干质量等都显著高于对照,菌根桑苗的叶绿素含量、光合速率、蒸腾速率、气孔导度显著高于非菌根桑苗.研究结果证明:桑苗对菌根真菌有着高度的依赖性,提高根系吸收能力,促进叶片光合作用,接种AM真菌能显著地促进桑苗根系和枝叶的生长,获得健壮的菌根化桑苗.
The rhizosphere of mulberry in the rocky desertification regions has diverse arbuscular mycorrhiza fungi （AMF）. In an investigation reported in this paper, three genera of AMF （Glornus, Acaulospora and Gigarspora） with sixteen species were identified according to their morphological characteristics, of which Acaulospora and Glomus were dominant genera around the rhizosphere of mulberry. Virus-free mulberry seedlings which had grown in experimental ponds in a netted chamber for 2 mouths were inoculated with Glomus mosseae, Glomus intraradices and Acaulospora scrobiculata separately, and mycorrhizal colonization rate, mycorrhizal dependency, growth and photosynthetic characteristics of the inoculated and uninoculated seedlings were measured after cnoculation for 45 day. The results showed the most colonization rate of mulberry roots was （38.67 ±1.44）% and the most mycorrhizal dependency of the mulberry seedlings was （203.03±14.93）%. The inoculated mulberry seedlings grew significantly better than the uninoculated control in the respects of seedlings height, taproot length, leaves per plant, area of the fifth leaf, fresh weight and dry weight of the plant. The content of chlorophyll net photosynthetic rate, transpi ration rate, stomatal conductance of AMF-inoculated mulberry were significantly higher than those of non- AMF-inoculated plants. The above research results confirmed that the inoculants mulberry was highly dependent on AMF. AMF significantly enhanced the root absorption capacity, improved leaf photosynthesis and promoted the growth and development of the seedlings. A lot of vigorous seedlings of mulberry mycorrhizaed were harvested in the experimental ponds.
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第10期24-30,共7页
Journal of Southwest University(Natural Science Edition)
基金
重庆市科技攻关重点项目(CSTC
2011AB1028)
现代农业产业技术体系专项(CARS-22-ZJ0503)基金资助
关键词
石漠化
AMF
多样性
桑树
壮苗
rocky desertification
AMF
diversity
mulberry
vigorous seedling
