摘要
目的建立柱前衍生-高效液相色谱法测定酒中氰化物含量的方法。方法采用异烟酸-巴比妥酸柱前衍生,高效液相色谱法测定。采用菲罗门Gemini C18(4.6 mm×250 mm,5μm)色谱柱,以1%乙酸-乙腈(50:50)为流动相,流速为1.0 ml/min,检测波长为600 nm。结果氰化物的衍生产物的线性范围为0.5~40μg/L,相关系数大于0.9998。仪器检出限为0.5μg/L,白酒、啤酒、葡萄酒中氰化物的平均回收率分别为96.7%,95.7%,96.3%(n=9)。结论该方法准确可靠、灵敏度高、稳定性好,有较好的样品适用性,可为酒中氰化物的测定标准提升提供参考。
Objective To establish a pre-column derivatization HPLC method for the determination of cyanide in liquor.Methods An HPLC method was applied,using isonicotinic acid-barbituric acid as precolumn derivatization agent.Phenomenex Gemini C18(4.6 mm×250 mm,5μm)column was adopted,using 1%acetic acid-acetonitrile(50:50)at the flow rate of 1.0 ml/min as mobile phase.The detection wavelength was set at 600 nm.Results The calibration curve was linear over the concentration range of 0.5-40μg/L for cyanide derivatives.The correlation coefficients of cyanide derivatives were greater than 0.9998.The limit of detection was 0.5μg/L.The average recoveries of cyanide were 96.7%for white spirits,95.7%for beer and 96.3%for wine(n=9).Conclusion The developed HPLC method is accurate,sensitive,stable and has good sample applicability.It can supply reference for improvement of the determination standard of cyanide in liquor.
作者
何维为
徐灿辉
何超
孙诗程
HE Wei-wei;XU Can-hui;HE Chao;SUN Shi-cheng(Yiyang Institute for Food and Drug Control,Yiyang 413000,China)
出处
《食品与药品》
CAS
2021年第2期128-132,共5页
Food and Drug
基金
益阳市科技计划项目(编号:2019YH14)。
