摘要
为探究模拟茶叶渣中关键成分制成的复合激活剂,提高两种不同混合菌群DDMY1、DDMY2对活性艳蓝19(reactive brilliant blue 19,RB19)脱色降解的机理,通过液相色谱质谱联用仪、高通量测序、非标及定量蛋白质组学进行分析。试验结果表明,经过72 h的培养,复合激活剂使菌群DDMY1的脱色率提高了7.24%,而菌群DDMY2的脱色率仅提高了1.77%,其对菌群DDMY1的促进作用明显高于对菌群DDMY2的作用。同时,激活剂能够富集肠杆菌属,这可能是增强混合菌群对RB19脱色降解的原因。在激活剂诱导下两种不同混合菌群DDMY1、DDMY2差异蛋白参与的KEGG(Kyoto Encyclopedia of Genes and Genomes)通路主要为代谢途径、次生代谢产物的生物合成、抗生素的生物合成、碳代谢。除上述的通路外,苯甲酸的降解途径也得到了证实。推测RB19被先降解为苯甲酸酯,随后进行糖酵解、柠檬酸循环等。研究结果对于激活剂强化染料生物降解机理研究具有一定指导意义。
In order to explore the mechanism of the complex activator developed by simulating key components of tea residue to improve the decolorization and degradation of RB19(reactive brilliant blue 19)by two bacterial consortium DDMY1 and DDMY2,the liquid chromatograph mass spectrometer,high-throughput sequencing,Label-free Proteomics analysis were adopted.The test results showed that after 72 hours of cultivation,the complex activator increased the decolorization rate of the bacterial consortium DDMY1 by 7.24%,while the decolorization rate of the bacterial consortium DDMY2 was only increased by 1.77%,and its promotion effect on the bacterial consortium DDMY1 was significantly higher than that of bacterial consortium DDMY2.At the same time,the activator could enrich Enterobacter,which may be the reason for enhancing the decolorization and degradation of RB19 by bacterial consortia.The first four KEGG(Kyoto Encyclopedia of Genes and Genomes)pathways of two different bacterial consortia DDMY1 and DDMY2 induced by the complex activator were metabolic pathways,biosynthesis of secondary metabolites,biosynthesis of antibiotics and carbon metabolism.In addition to the above pathways,the degradation pathway of benzoic acid was also abundant.It could be speculated that RB19 was firstly degraded to benzoate,and then participated in glycolysis,citric acid cycle,etc.The results have certain guiding significance for the study on the mechanism of activator-enhanced dye biodegradation.
作者
王怡琴
谢学辉
郑秀林
张庆云
从军灏
刘娜
柳建设
WANG Yiqin;XIE Xuehui;ZHENG Xiulin;ZHANG Qingyun;CONG Junhao;LIU Na;LIU Jianshe(College of Environmental Science and Engineering,Donghua University,Shanghai 201620,China;Textile Pollution Controlling Engineering Center of Ministry of Envionmental Protection,Donghua University,Shanghai 201620,China;Shanghai Institute of Pollution Control and Ecological Security,Shanghai 200092,China;School of Environmental and Surveying Engineering,Suzhou University,Suzhou 234000,China)
出处
《东华大学学报（自然科学版）》
CAS
北大核心
2021年第2期73-83,共11页
Journal of Donghua University(Natural Science)
基金
国家重点研究发展计划资助项目(2019YFC0408304)
中央高校基本科研业务费专项资金资助项目(2232019D322)
安徽省自然科学基金资助项目(1808085QE176)。
关键词
复合激活剂
蒽醌染料生物降解
蛋白质组学
混合菌群
降解路径
高通量测序
complex activator
anthraquinone dye biodegradation
proteomics
bacterial consortium
degradation pathway
high-throughput sequencing
