摘要
目的探究双孔钾离子通道(TRESK)介导的mTOR信号通路对大鼠脊髓神经元细胞凋亡的影响。方法培养100例SD大鼠的脊髓原代神经元细胞,分为4组,每组各25例,A组采用TRESK siRNA、mTOR特异性抑制剂、谷氨酸处理,B组采用mTOR特异性抑制剂、谷氨酸处理,C组不进行任何处理,D组仅进行谷氨酸处理。对比各组24 h后的脊髓神经元凋亡蛋白水平、脊髓神经元活力、脊髓神经元细胞凋亡率、测定mTOR磷酸化水平及TRESK mRNA表达水平。结果A组、B组、D组的cleaved Caspase3水平和Bax水平均高于C组,差异有统计学意义(P<0.05),B组的cleaved Caspase3和Bax水平最高,与其他组比较,差异有统计学意义(P<0.05)。与C组比较,A、B、D组SD大鼠脊髓神经元活力均降低,细胞凋亡率均升高(P<0.05);与C组比较,A、B、D组mTOR磷酸化水平表达、TRESK mRNA表达均降低(P<0.05)。结论由TRESK介导的mTOR信号通路在抑制谷氨酸诱导的脊髓神经元凋亡中产生一定作用。
Objective To research the impacts of TRESK(double-pore potassium channel)mediated mTOR signaling pathway on apoptosis of spinal cord neurons in rats.Methods A total of 100 primary spinal cord neurons of SD rats were cultured and divided into group A,group B,group C and group D,25 rats in each group.Group A was treated with TRESK siRNA,mTOR specific inhibitor,glutamate,Group B was treated with mTOR specific inhibitor,glutamate,Group C was given no treatment,and group D was treated with glutamic acid only.The levels of apoptotic protein,vitality and apoptosis rate of spinal cord neurons were compared after 24 h in each group,and the expression levels of mTOR phosphorylation and TRESK mRNA were measured.Results The levels of cleaved Caspase 3 and Bax in group A,group B and group D were higher than those in group C,and the differences were statistically significant(P<0.05).The levels of cleaved Caspase 3 and Bax in group B were the highest,and the differences were statistically significant compared with other groups(P<0.05).Compared with group C,the vitalities of spinal cord neurons of SD rats in group A,B and D were decreased and the apoptosis rates were increased(P<0.05).Compared with group C,the expressions of mTOR phosphorylation levels and TRESK mRNA in group A,B and D were all decreased(P<0.05).Conclusion The TRESK mediated mTOR signaling pathway plays a certain role in inhibiting glutamate-induced apoptosis of spinal cord neurons.
作者
王文康
李恒昌
陈廷凤
WANG Wenkang;LI Hengchang;CHEN Tingfeng(Department of Anesthesiology,Guangzhou First People's Hospital,Guangdong,Guangzhou 510180,China)
出处
《中国医药科学》
2021年第5期32-35,共4页
China Medicine And Pharmacy
基金
广东省广州市医药卫生科技项目(20161A011004)。
