摘要
目的:探讨环境雌激素样饲料联合冷刺激诱导的ED大鼠血清与阴茎组织中促炎因子IL-6、TNF-α和TGF-β1的变化及其对ED大鼠阴茎组织纤维化的影响。方法:取100只SD雄性大鼠,20只为正常组(N),其余120只为建模组,以环境雌激素样饲料联合冷刺激予以复合干预,24周后通过APO阴茎勃起实验和交配实验筛选ED大鼠,并从中随机选取20只大鼠为勃起功能障碍组(ED组),以ELISA和Western blot法分别检测各组大鼠血清和阴茎组织中IL-6、TNF-α和TGF-β1表达水平,Masson染色观察阴茎组织纤维化情况。结果:(1)ED组大鼠血清中IL-6、TNF-α和TGF-β1含量较N组显著升高(P<0.05);(2)ED组大鼠阴茎组织中IL-6、TNF-α和TGF-β1蛋白相对表达水平较N组显著升高(P<0.05);(3)ED组大鼠阴茎组织平滑肌/胶原纤维比值显著低于N组(P<0.05)。结论:环境雌激素样饲料联合冷刺激建立的复合应激型ED大鼠阴茎组织发生纤维化是其发生ED的重要组织病理学原因之一,具体机制可能和血清和组织中促炎细胞因子高表达密切相关。
Objective:To explore the changes of pro-inflammatory factors IL-6,TNF-αand TGF-β1 in the serum and penile tissue of ED rats induced by environmental estrogen-like diet combined with cold stimulation and their effects on the fibrosis of the penile tissue of ED rats.Methods:120 male Sprague-Dawley rats were randomly divided into 2 groups:normal control group(N,n=20)were feeding normal environment and diet,and model group(n=100)were intervened in damp and cold environment and gave estrogen-like feed to model for 24 w to screen ED rats,and then ELISA and Western-blot methods were used to detect the expression levels of IL-6,TNF-αand TGF-β1 in the serum and penile tissues of rats in each group,observe the fibrosis of penile tissues by Masson staining.Results:(1)IL-6,TNF-αand TGF-β1 in ED rats serum were significantly increased compared with N group(P<0.05);(2)The relative expression levels of IL-6,TNF-αand TGF-β1 protein in the penile tissue of the ED group were significantly higher compared with N group(P<0.05);(3)The penile tissue smooth muscle/collagen fiber ratio of the ED group was significantly lower compared with N group(P<0.05).Conclusion:The fibrosis of the penile tissue of compound stress ED rats induced by environmental estrogen-like diet combined with cold stimulation is one of the important histopathological reasons for the occurrence of ED.The specific mechanism may be closely related to the high expression of pro-inflammatory cytokines in serum and tissues.
作者
马文静
斯依提·阿木提
阿地力江·伊明
刘凤霞
侯鹏程
MA Wen-jing;SIYITI·A-muti;ADILIJIANG·Yi-ming;LIU Feng-xia;HOU Peng-cheng(Central Laboratory,Xinjiang Medical University,Urumqi,Xinjiang,830011,China;Department of Human Anatomy,College of Basic Medicine,Xinjiang Medical University,Urumqi,Xinjiang,830011,China)
出处
《现代生物医学进展》
CAS
2021年第5期825-829,共5页
Progress in Modern Biomedicine
基金
新疆维吾尔自治区自然科学基金项目(2017D01C239)。
关键词
促炎细胞因子
冷刺激
环境雌激素
勃起功能障碍
纤维化
Proinflammatory cytokines
Cold stress
Environmental estrogen
Erectile dysfunction
Fibrosis
