摘要
目的研究痰热清注射液分别联合头孢他啶、头孢哌酮钠舒巴坦钠、哌拉西林钠他唑巴坦钠及亚胺培南西司他丁钠对耐碳青霉烯鲍曼不动杆菌的体外抑菌作用。方法选用微量肉汤稀释法测定痰热清注射液、头孢他啶、头孢哌酮钠舒巴坦钠、哌拉西林钠他唑巴坦钠及亚胺培南西司他丁钠单用及联合应用对重症监护室(ICU)的30株耐碳青霉烯鲍曼不动杆菌最低抑菌浓度(MIC),计算部分抑菌浓度(FIC)指数,评价药物联合应用的抑菌效果。结果痰热清注射液单用抑菌MIC范围为稀释至原液的1/4~1/2,联用后痰热清的MIC范围、MIC50和MIC90均明显降低。痰热清联合头孢他啶的FIC指数为:FIC≤0.5占13.3%,0.5<FIC≤1占66.7%,1<FIC≤4占20.0%;痰热清联合头孢哌酮钠舒巴坦钠的FIC指数为:FIC≤0.5占16.7%,0.5<FIC≤1占83.3%;痰热清联合哌拉西林钠他唑巴坦的FIC指数为:FIC≤0.5占3.3%,0.5<FIC≤1占90.0%,1<FIC≤4占6.7%;痰热清联合亚胺培南西司他丁钠的FIC指数为:FIC≤0.5占10.0%,0.5<FIC≤1占80.0%,1<FIC≤4占10.0%。结论痰热清注射液单用对体外的耐碳青霉烯鲍曼不动杆菌有一定抑菌作用,其分别联合四种抗菌药物各药液最低浓度均不同程度的减少,头孢哌酮钠舒巴坦钠和哌拉西林钠他唑巴坦以协同和相加作用为主,抑菌效果较好。头孢他啶无关作用明显,抑菌作用较差。
Objective To research the bacteriostasis effect of Tanreqing Injection combined with ceftazidime,cefoperazone sodium and sulbactam sodium,piperacillin sodium tazobactam sodium and imipenem cilastatin sodium on carbapenem-resistant Acinetobacter baumannii in vitro,respectively.Methods The minimal inhibitory concentration(MIC)of Tanreqing Injection,ceftazidime,cefoperazone sodium,sulbactam sodium,piperacillin sodium tazobactam sodium and imipenem cilastatin sodium on 30 strains of carbapenem-resistant Acinetobacter baumannii in intensive care unit(ICU)when alone and combined were determined by micro-broth dilution method,and the fractional inhibitory concentration(FIC)index was calculated to evaluate the bacteriostasis effect of combined drug application.Results The bacteriostatic MIC range of Tanreqing Injection alone was diluted to 1/4-1/2 of the stock solution,and the MIC range,MIC50 and MIC90 of Tanreqing Injection were significantly reduced after combined use.The FIC index of Tanreqing combined with ceftazidime was as follows:FIC≤0.5 accounted for 13.3%,0.5<FIC≤1 accounted for 66.7%,and 1<FIC≤4 accounted for 20.0%;The FIC index of Tanreqing combined with cefoperazone sodium and sulbactam sodium was as follows:FIC≤0.5 accounted for 16.7%,and 0.5<FIC≤1 accounted for 83.3%;The FIC index of Tanreqing combined with piperacillin sodium tazobactam was as follows:FIC≤0.5 accounted for 3.3%,0.5<FIC≤1 accounted for 90.0%,and 1<FIC≤4 accounted for 6.7%;The FIC index of Tanreqing combined with imipenem and cilastatin sodium was as follows:FIC≤0.5 accounted for 10.0%,0.5<FIC≤1 accounted for 80.0%,and 1<FIC≤4 accounted for 10.0%.Conclusion Tanreqing Injection alone has a certain bacteriostasis effect on carbapenem-resistant Acinetobacter baumannii in vitro,and the minimum concentration of each solution combined with four acinetobacter drugs is reduced to varying degrees.Cefoperazone sodium,sulbactam sodium and piperacillin sodium and tazobactam have mainly synergistic and additive effects,and the bacteriostasis effect is better.Ceftazidime has no significant effect,and its bacteriostasis effect is poor.
作者
刘佳
陶庆春
李公民
赵玉玲
程丽娟
LIU Jia;TAO Qingchun;LI Gongmin;ZHAO Yuling;CHENG Lijuan(Department of Clinical Laboratory,Beijing Fangshan Traditional Chinese Medicine Hospital,Beijing 102400,China;Department of Clinical Laboratory,the Third Hospital Affiliated to Beijing University of Chinese Medicine,Beijing 100029,China;Department of Clinical Laboratory,Beijing Yanhua Hospital,Beijing 102500,China;Department of Clinical Laboratory,Beijing Fangshan Liangxiang Hospital,Beijing 102401,China)
出处
《中国医药科学》
2021年第7期9-12,共4页
China Medicine And Pharmacy
基金
北京市房山区优秀人才培养资助青年骨干个人项目(2018000000007G016)。
关键词
痰热清
耐碳青霉烯鲍曼不动杆菌
抑菌作用
Tanreqing
Carbapenem-resistant Acinetobacter baumannii
Bacteriostasis effect
