摘要
试验旨在探究油酸对延边牛骨骼肌卫星细胞成脂转分化的影响。试验设1个空白对照组(CON)和3个油酸(OA)诱导组:50、100、200μmol/L油酸组(OAL、OAM、OAH)。油酸诱导96 h后,通过测定细胞大小及活力评估油酸对细胞的影响。通过油红O染色和测定甘油三酯来验证脂滴的形成,通过实时荧光定量PCR测定相关成肌成脂基因的表达水平来验证脂肪细胞的生成。结果显示,与对照组相比,添加油酸后,延边牛骨骼肌卫星细胞内有脂滴生成,并且脂滴形成量、甘油三酯累积量与油酸呈剂量依赖关系;实时荧光定量PCR测定结果表明,成肌相关因子Pax3、MyoD显著下调(P<0.05),成脂相关因子C/EBPβ、PPARγ显著上调(P<0.05),脂肪酸代谢相关因子SCD显著下调(P<0.05),PLIN2基因显著上调(P<0.05)。综合上述试验结果,用油酸诱导处理延边牛骨骼肌卫星细胞可促进细胞的成脂转分化。
The present study was aimed to explore the effect of oleic acid on the adipogenic transdifferentiation of Yanbian bovine skeletal muscle satellite cells.The experiment was set up 1 blank control group(CON)and 3 oleic acid(OA)induction groups:50,100 and 200μmol/L oleic acid groups(OAL,OAM and OAH).96 h after oleic acid induction,the effect of oleic acid on cells was evaluated by measuring cell size and viability.Oil red O staining method and triglyceride determination were used to verify the formation of lipid droplets,the expressions of related myogenic adipogenic genes were measured by Real-time quantitative PCR to verify the production of adipocytes.The results showed that,compared with the control group,when oleic acid was added,lipid droplets were formed in Yanbian cattle skeletal muscle satellite cells,and the amount of lipid droplet formation and trigbyceride accumulation were dose-dependent with oleic acid.Real-time PCR measurement results showed that the myogenic related factors Pax3 and MyoD were significantly down-regulated(P<0.05),the adipogenic factors C/EBPβand PPARγwere significantly up-regulated(P<0.05),the fatty acid metabolism-related factor SCD was significantly down-regulated(P<0.05),and the PLIN2 gene was significantly up-regulated(P<0.05).The results of the experiment showed that the induction and treatment of Yanbian bovine skeletal muscle satellite cells with oleic acid could promote the adipogenic transdifferentiation of the cells.
作者
孙建富
张军芳
孙斌
王英
崔岩
李强
CHOI Seong H
SHIN Jong S
李香子
SUN Jianfu;ZHANG Junfang;SUN Bin;WANG Ying;CUI Yan;LI Qiang;CHOI Seong H;SHIN Jong S;LI Xiangzi(Northeast Cold Region Beef Cattle Science and Technology Innovation Ministry of Education Engineering Research Center,Yanbian University, Yanji 133002,China;College of Animal Life Sciences,Chungbuk National University,Cheongju 361-763,South Korea;College of Animal Life Sciences,Gangwon National University,Chuncheon 200-701,South Korea)
出处
《中国畜牧兽医》
CAS
北大核心
2021年第4期1188-1195,共8页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金“PLIN2在油酸诱延边黄牛骨骼肌细胞向脂肪细胞分化的作用及其机制研究”(31860653)
高等学校学科创新引智计划(D20034)。
关键词
油酸
延边牛
骨骼肌卫星细胞
成脂
oleic acid
Yanbian cattle
skeletal muscle satellite cells
adipogenesis
