摘要
为了调查2015—2016年中国部分地区猪博卡病毒流行情况,在我国各地中抽取了89份猪样本,用PCR方法对这些样本进行检测,并对其中有扩增阳性的产物进行基因序列检测,将测序结果进行对比分析,找出其中可能存在的同源性或遗传性证据。在89份检测样本中,检测出PBoV的样本42分,占比47.19%,其中山东地区的样本阳性率最低,上海、江西地区阳性率最高。NS1基因序列分析结果显示,鉴定的5株PBoV均属于PBoV 3型,且这5株PBoV的核苷酸同源性达到了80.0%~85.6%,因此,可以预测其氨基酸的同源性在82.9%至87.9%之间。将这5株进行遗传进化分析,结果显示:检测的MH822023、MH822025、MH822024、MH82022、MH82026毒株与参考KC473563、KF025390、KF025379、KF025389、KF025380毒株具有较高同源性。
In order to investigate the prevalence of boca virus(PBoV)in some regions of China from 2015 to 2016,89 samples of pigs were collected from some parts of China.These samples were tested by PCR,and the products with positive amplification were detected by gene sequences.The sequencing results were compared and analyzed to find the possible evidence of homology or heredity.Among the 89 test samples,42 points(47.19%)of PBoV samples were detected,with the lowest positive rate in Shandong province and the highest in Shanghai and Jiangxi province.The results of NS1 gene sequence analysis showed that all the five identified PBoV strains belonged to PBoV type 3,and the nucleotide homology of these five PBoV strains reached 80.0%~85.6%,so the homology of its amino acids could be predicted to between 82.9%and 87.9%.Genetic and evolutionary analysis of the five strains showed that the detected MH822023,MH822025,MH822024,MH82022 and MH82026 strains had high homology with the reference strains KC473563,KF025390,KF025379,KF025389 and KF025380.
作者
许雅茹
苏明俊
孙东波
Xu Yaru;Su Mingjun;Sun Dongbo(Heilongjiang Bayi Agricultural University,Daqing163319)
出处
《黑龙江八一农垦大学学报》
2021年第2期17-25,共9页
journal of heilongjiang bayi agricultural university
基金
科研团队支持计划猪病防制创新团队(TDJH201804)
黑龙江省博士后科研启动基金项目(LBH-Q16188)
黑龙江省杰出青年科学基金项目(JC2017007)
黑龙江省农垦总局科技攻关项目(HNK135-04-06-03)兽医科学。
