摘要
多细胞有机体的细胞类型多且复杂,细胞间普遍存在异质性。目前,单细胞转录组测序(single-cell RNA sequencing,scRNA-seq)技术是一项新兴的研究单个细胞转录水平的技术,其从数千个平行的细胞中生成转录谱,揭示个体细胞基因组的差异性表达,反映细胞间的异质性,从而鉴定出不同细胞类型,形成组织或器官的细胞图谱,在生物和临床医学等领域发挥重要作用。该文在对scRNA-seq测序平台进行阐述和比较的基础上,着重介绍其在神经系统和免疫系统细胞类型探索中的应用,并且总结scRNA-seq与空间转录组技术相结合的研究成果。
The cell types of multicellular organisms are numerous and complex,and inter-cell heterogeneity is prevalent.The emerging scRNA-seq(single-cell RNA sequencing)technology is of great importance in researching the transcription levels of individual cells.This technology produces a transcription profile from thousands of parallel cells,reveals the differential expression of single cell genome,and reflects the heterogeneity between cells.This technology identifies different types of cells and forms a cell map of tissues or organs.Having a significant role in bioscience and clinical medicine,scRNA-seq technology is becoming a focal point of life science research.Based on the detailed description and comparison of scRNA-seq sequencing platform,this review focuses on the exploration of cell types in nervous system and immune system,and summarizes the application of scRNA-seq and space transcriptome technology.
作者
杨佳凤
陈鹏璐
龚熹
YANG Jiafeng;CHEN Penglu;GONG Xi(School of Life Science,Nanchang University,Nanchang 330031,China)
出处
《中国细胞生物学学报》
CAS
CSCD
2021年第2期476-483,共8页
Chinese Journal of Cell Biology
基金
国家自然科学基金(批准号:31960167)资助的课题。
