摘要
目的:探讨中药五味子中五味子丙素的体外抗胃癌活性。方法:在胃癌细胞AGS中,采用MTT法和集落形成实验测定五味子丙素对细胞的生长抑制活性,采用流式细胞仪测定其诱导细胞凋亡的活性,采用Western blot法测定其对凋亡蛋白表达的影响。并用MTT法测定其对不良反应较大的传统胃癌一线化疗药物5-FU的增敏活性。结果:五味子丙素对胃癌细胞AGS生长抑制的IC50为(35.73±0.28)μmol/L,并能浓度剂量依赖性地抑制细胞集落形成,诱导细胞凋亡,上调凋亡蛋白Cleaved-PARP,下调抗凋亡蛋白Bcl-2的表达(P<0.05)。结论:五味子丙素对胃癌细胞具有较好的抑制生长和诱导凋亡的活性。
Objective:To investigate in vitro anti-gastric cancer activity of schisandrin C.Methods:MTT assay and colony formation assay were used to examine the growth inhibitory activity of schisandrin C in AGS cells.After treatment with different concentrations of schisandrin C,the apoptosis of AGS cells was detected by flow cytometer.Meanwhile,Western blot was performed to detect the expression of apoptotic proteins.5-FU,a traditional first-line chemotherapy drug for gastric cancer,MTT assay was used to detect the synergism with 5-FU.Results:The half maximal inhibitory concentration(IC50)of schisandrin C against AGS cells was(35.73±0.28)μmol/L,which could inhibit cell colony formation,induce cell apoptosis,up-regulate the apoptotic protein Cleaved-PARP,and down-regulate the expression of anti-apoptotic protein Bcl-2 in a dose-dependent manner.Conclusion:Schisandrin C has stronginhibitory activity and induces apoptosis on gastric cancer cells.
作者
姚佳丽
徐海能
范齐昀
董昭骏
吴建章
YAO Jiali;XU Haineng;FAN Qiyun;DONG Zhaojun;WU Jianzhang(Chemical Biology Research Center,School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou 325035,China)
出处
《温州医科大学学报》
CAS
2021年第3期187-191,共5页
Journal of Wenzhou Medical University
基金
国家自然科学基金资助项目(81703766)。
关键词
五味子丙素
胃癌
细胞凋亡
schisandrin C
gastric cancer
apoptosis
