摘要
目的:探讨五味子乙素(Sch B)作用于脑胶质瘤SHG-44细胞后对血管内皮生长因子(VEGF)表达的影响及细胞生长抑制作用,阐明其可能机制。方法:培养脑胶质瘤SHG-44细胞,将其分为对照组和Sch B50、100、200mg.L-1组,MTT法检测不同剂量Sch B作用SHG-44细胞后其生长情况;酶联免疫吸附实验和细胞免疫组织化学方法观察Sch B作用后SHG-44细胞VEGF蛋白表达情况。结果:与对照组比较,200mg.L-1 Sch B作用SHG-44细胞24h,细胞增殖活性轻度增加(P<0.05);Sch B(50、100和200mg.L-1)作用48或72h,细胞增殖活性明显降低(P<0.05或P<0.01);200mg.L-1 Sch B作用SHG-44细胞72h,培养上清中VEGF蛋白表达水平明显降低(P<0.01)。与对照组比较,Sch B作用SHG-44细胞72h后,Sch B 50、100和200mg.L-1组VEGF蛋白阳性表达率均明显降低(P<0.01)。结论:Sch B能明显抑制SHG-44细胞VEGF蛋白表达及细胞生长,提示Sch B可能通过抑制脑胶质瘤血管生成而发挥抗肿瘤作用。
Objective To explore the effect of schisandrin B （Sch B） on expression of vascular endothelial growth factor （VEGF） in glioma SHG-44 cells and its inhibitory effect on cell growth and to explore the possible mechanism. Methods Glioma SHG-44 cells were separated to control and 3 treatment groups, and then treated with 0, 50, 100 and 200 mg ~ L-1 Sch B for 24--72 h. MTT assay was used to evaluate the proliferation of glioma SHG-44 cells. The levels of VEGF protein expression after treated with Sch B were measured by both ELISA and immuncytochemistry. Results Compared with control group , the proliferation activity of glioma SHG-44 cells was slightly increased after treated with 200 mg L-a Sch B for 24 h （P〈0.05） ; the proliferation abilities of the SHG-44 ceils were obviously decreased after treated with 50, 100 and 200 rag L-1 Sch B for 48 h or 72 h; the VEGF protein level in the supernatant of cultured cells after treated with Sch B for 72 h was significantly decreased （P〈0. 01）. After the glioma SHG-44 cells were treated with Sch B for 72 h, the positive expression rates of VEGF protein in 50, 100 and 200 mg L-1 groups were markedly reduced compared with control group （P〈 0.01）. Oonclusion Sch B can inhibit the expression of VEGF protein and growth of glioma SHG-44 cells, it suggests that Seh B may exert anti-glioma effect by inhibiting angiogenesis in glioma.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2013年第1期1-4,共4页
Journal of Jilin University：Medicine Edition
基金
国家自然科学基金资助课题(81201671)
吉林省教育厅基金资助课题(2011281
2012330)
