摘要
目的探讨声诺维与脂质体两种靶向载体负载尿激酶的优缺点,找出最适宜的载体。方法分别制备靶向血栓载尿激酶的声诺维和脂质体,并检测各自的以下指标:声诺维测粒径,用流式细胞仪检测其与精氨酸-甘氨酸-天冬氨酸序列RGD、尿激酶的双结合率;脂质体测粒径,测载药量与包封率,并绘制尿激酶的体外释放曲线。结果靶向血栓载尿激酶声诺维粒径约4432.7 nm,洗涤前双结合率为76.33%,洗涤后双结合率为46.91%;靶向血栓载尿激酶脂质体粒径约141.3 nm,载药量与包封率分别约为19.07%、34.33%。结论靶向血栓载尿激酶的声诺维粒径较大,属微米级别,三者结合不稳定,洗涤后双结合率明显下降;靶向血栓载尿激酶脂质体粒径小,属纳米级别,目前制备的脂质体包封率和载药量不高,有提高的空间。
Objective To explore the advantages and disadvantages of SonoVue and liposomes for loading urokinase,and find the most suitable carrier.Methods Carriers of SonoVue and liposomes targeting thrombus containing urokinase were prepared,and the following indicators of each were detected.Sonovi to measure the particle size,its double binding rate with arginine-glycine-aspartic acid Sequence(RGD)and urokinase was detected by flow cytometry.Liposomes to measure particle size,drug loading and encapsulation rate,and the in vitro release curve of urokinase was drawn.Results The particle size of SonoVue targeted thrombus containing urokinase was about 4432.7 nm,the double binding rate was 76.33%before washing and 46.91%after washing.The size of liposomes loaded with urokinase was about 141.3 nm,the drug loading and encapsulation rate were about 19.07%and 34.33%,respectively.Conclusion The particle size of SonoVue targeted thrombus containing urokinase is large,which belongs to micron level,and the combination of the three is unstable,and the double binding rate is significantly reduced after washing.The particle size of liposomes targeting thrombus containing urokinase is small and is of nanometer level,but the disadvantage is that the encapsulation rate and drug loading of the prepared liposomes are not high at present.
作者
饶华清
车雪瑜
潘晓熊
黄岗
陈星
吴棘
RAO Huaqing;CHE Xueyu;PAN Xiaoxiong;HUANG Gang;CHEN Xing;WU Ji(Graduate School of Guangxi Medical University,Nanning,Guangxi 530021,China;Guangxi Academy of Sciences,Nanning,Guangxi 530003,China)
出处
《重庆医学》
CAS
2021年第5期845-849,共5页
Chongqing medicine
基金
国家自然科学基金项目(81760314)
广西医学高层次骨干人才139计划(G201903014)。
