摘要
目的观察人源脐带间充质干细胞(hUC-MSCs)对异体总淋巴细胞和不同淋巴细胞亚群增殖能力的影响,以及对细胞因子分泌的影响。方法分离并培养hUC-MSCs及健康成人外周血淋巴细胞,以淋巴细胞为阴性对照,MTT法检测共培养(淋巴细胞∶hUC-MSCs为1∶0.5、1∶1、1∶5、1∶10)3 d后淋巴细胞的增殖情况;流式细胞仪检测共培养体系中CD4^(+)CD25^(+)FOXP3^(+)、CD3-CD16^(+)CD56^(+)、CD4^(+)CD45RA^(+)CCR7^(+)的细胞比例;ELISA法检测白细胞介素(IL)-2、IL-4、IL-10、γ-干扰素(INF-γ)细胞因子分泌情况。结果与阴性对照组比较,hUC-MSCs对淋巴细胞的增殖具有显著抑制作用(P<0.05),抑制效果随数量增加而增强;hUC-MSCs能够显著增加共培养体系中CD4^(+)CD25^(+)FOXP3^(+)细胞比例(P<0.05),显著降低CD4^(+)CD45RA^(+)CCR7^(+)细胞比例(P<0.05),对CD3^(-)CD16^(+)CD56^(+)细胞比例无显著影响;hUC-MSCs能够显著降低IL-2、IL-4、INF-γ水平,显著增加IL-10的分泌水平(P<0.05)。结论hUC-MSCs具有免疫抑制功能。
Objective To observe the effects of human umbilical cord mesenchymal stem cells(hUC-MSCs)on the proliferation of allogeneic total lymphocytes and different lymphocyte subsets,as well as the secretion of cytokines.Methods hUC-MSCs and peripheral blood lymphocytes of healthy adults were isolated and cultured;MTT method was used to detect the proliferation of lymphocytes by after co-culture for 3 days;the proportion of CD4^(+)CD25^(+)FOXP3^(+),CD3^-CD16^(+)CD56^(+)and CD4^(+)CD45RA^(+)CCR7^(+)in the co-culture system was detected by flow cytometry;ELISA was used to detect the secretion of IL-2,IL-4,IL-10 and INF-γ.Results hUC-MSCs could significant inhibit the proliferation of T lymphocytes,and the anti-proliferative effect was enhanced with the increase of the number of lymphocytes;the proportion of CD4^(+)CD25^(+)FOXP3^(+)cells in co-culture system was increased by hUC-MSCs(P<0.05);the proportion of CD4^(+)CD45RA^(+)CCR7^(+)cells showed a downward tendency,and the difference was statistically significant(P<0.05);however,there was no significant difference in CD3^(-)CD16^(+)CD56^(+)cells.hUC-MSCs could reduce the levels of IL-2,IL-4 and INF-γ,but increase the secretion level of IL-10,and the difference was statistically significant(P<0.05).Conclusion HUC-MSCs had immunosuppressive function.
作者
杨莹
李政楠
王秀娟
李伟
牟春琳
金星
徐永胜
YANG Ying;LI Zhengnan;WANG Xiujuan;LI Wei;MU Chunlin;JIN Xing;XU Yongsheng(Ever Union Biotechnology Company Limited,Tianjin 301925,China)
出处
《药物评价研究》
CAS
2020年第12期2385-2389,共5页
Drug Evaluation Research
基金
国家重点研发计划“干细胞及转化研究”重点专项(2019YFA0112100)
天津市科技计划项目(17ZXXYSY00020)。
关键词
脐带间充质干细胞
淋巴细胞
免疫反应
细胞因子
umbilical cord mesenchymal stem cells
lymphocytes
immune response
cytokines
