摘要
随着诱导多能干细胞技术的建立和发展,不同物种的诱导多能干细胞被相继报道。诱导多能干细胞的核型鉴定方法对于日常监控染色体突变显得日益重要。本文建立了人、大鼠和猪诱导多能干细胞的核型鉴定方法,同时分析了秋水仙素浓度、作用时间和低渗处理时间三个关键因素对诱导多能干细胞染色体核型分析成功的影响。结果显示低渗时间和秋水仙素的浓度不是核型分析成功的决定因素,关键因素是秋水仙素的作用时间,不同物种的诱导多能干细胞需要不同的处理时间。这为进一步建立其它新物种的诱导多能干细胞核型分析方法打下了基础。
After the establishment and development of induced pluripotent stem cell （iPS cell） technique, the iPS cells from various species have been reported. To monitor the mutation of chromosomes in iPS cells, the karyotype analysis became more and more important. We established the protocol to do karyotyping for human, pig and rat iPS cells. We also investigated the factors involved in the quality of the karyotyping including colchicine concentration, duration of colchicine treatment and duration of hypotension. We demonstrated that the key factor in this method is duration of colchicine treatment. Our report should facilitate the establishment and studies of iPS cells of new species.
出处
《细胞生物学杂志》
CSCD
2009年第3期443-447,共5页
Chinese Journal of Cell Biology
关键词
诱导多能干细胞
核型分析
染色体
induced pluripotent stem cell
karyotype analysis
chromosome
