摘要
呼吸道感染性疾病是全球成人和儿童发病和死亡的重要原因,其病原体的诊断非常重要,并且是难点问题。现行的临床病原学检测的方法中,传统方法包括涂片染色镜检法、抗原检测法、培养法等。对于阳性的培养物还可以用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)进行菌种鉴定。最近几年发展较快的是分子生物学检测方法,包括普通聚合酶链反应(PCR)、多重PCR、实时定量PCR等和宏基因组测序(mNGS)技术。快速分子生物学检测技术,有望成为帮助指导靶向治疗和抗生素合理应用的有效工具。
Respiratory tract infections are the leading cause of morbidity and mortality in adults and children worldwide.The diagnosis of microbial pathogens of respiratory tract infections is very important and challenging.Am-ong the current clinical pathogen detection methods,the traditional methods include smear staining and microscopic examination,antigen detection,culture,etc.Matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS)can also be used for species identification of positive cultures.In recent years,molecular biological detection methods have developed rapidly,including common polymerase chain reaction(PCR),multiplex PCR,real-time quantitative PCR,and metagenomics next generation sequencing(mNGS)technology.Rapid and point-of-care testing(POCT)molecular methods are expected to be effective tools to help guide targeted therapy and appropriate use of antibiotics.
作者
胡晏宁
鲁炳怀
HU Yan-ning;LU Bing-huai(Peking University China-Japan Friendship School of Clinical Medicine,Beijing 100871,China)
出处
《中国临床新医学》
2021年第1期8-12,共5页
CHINESE JOURNAL OF NEW CLINICAL MEDICINE
基金
国家重点研发计划课题(编号:2018YFC1200100,2018YFC1200102)。
关键词
呼吸道感染
抗原检测
血清学检测
聚合酶链反应
基因组测序
Respiratory tract infection
Antigen identification
Serological identification
Polymerase chain reaction(PCR)
Metagenomic sequencing
