摘要
本试验旨在研究野生林麝(Moschus berezovskii)瘤胃、小肠和大肠微生物组成和抗生素抗性基因。采集野生林麝消化道3个区段(瘤胃、小肠和大肠)的内容物进行宏基因组测序,并进行常规物种注释和抗生素抗性基因功能注释。结果表明:3个区段共有优势菌门为厚壁菌门(Firmicutes)和变形菌门(Proteobacteria);瘤胃中主要优势菌属为普雷沃氏菌属(Prevotella)、月形单胞菌属(Selenomonas),小肠中主要优势菌属为链球菌属(Streptococcus)、埃希氏菌属(Echerichia),大肠中主要优势菌属为梭菌属(Clostridium)、拟杆菌属(Bacteroides)。基因常规注释显示各个区段微生物基因在不饱和脂肪酸合成和抗生素抗性上差异较大。抗生素抗性基因注释显示macB、sav1866和bcrA绝对丰度最高,且都来自于大肠细菌;瘤胃中抗生素抗性基因绝对丰度最大的是Aminocoumarin_resistant_alaS,小肠中是adeG,大肠中是macB。通过对野生林麝消化道微生物组成分区段比对,发现瘤胃、小肠和大肠微生物组成和抗生素抗性基因分布存在较大差异,大肠和小肠中细菌与野生林麝的多重耐药性关系更密切。
This experiment aimed to study the microbiota composition and antibiotic resistance genes(ARGs)in the rumen,small intestine and large intestine of wild forest musk deer(Moschus berezovskii).Metagenomic sequencing was used to measure the contents of three segments of digestive tract(rumen,small intestine and large intestine)of wild forest musk deer,then general species annotation and functional annotation of ARGs were performed.The results showed that the share dominant bacterial phyla in the three segments of digestive tract were Firmicutes and Proteobacteria;and the dominant bacterial genera in the rumen were Prevotell and Selenomonas,with Streptococcus and Echerichia in the small intestine,Clostridium and Bacteroides in the large intestine.Gene general annotation showed that there were significant differences in the unsaturated fatty acid synthesis and antibiotic resistance among the microbial genes in different digestive tract segments.ARGs annotation indicated that macB,sav1866 and bcrA had the highest absolute abundance,and all of them came from large intestine’s bacteria;the highest absolute abundance of ARGs in the rumen was Aminocoumarin_resistant_alaS,with adeG in the small intestine and macB in the large intestine.This paper makes a comparison of different digestive tract segments on the microbiota composition of the wild forest musk deer,it is preliminarily found that the microbiota composition and ARGs in the different segments of the digestive tract are quite different,and the bacteria in the large intestine and small intestine are more closely related to multidrug resistance of the wild forest musk deer.
作者
何森
曹新芳
郑雪莉
王豆
王洪永
蒋本模
卜书海
HE Sen;CAO Xinfang;ZHENG Xueli;WANG Dou;WANG Hongyong;JIANG Benmo;BU Shuhai(College of Life Science,Northwest A&F University,Yangling 712100,China;College of Forestry,Northwest A&F University,Yangling 712100,China;Fengxian Fengchun Jimin Credible Science and Technology Breeding Co.,Ltd.,Fengxian 721000,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2021年第1期484-493,共10页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
陕西省重点研发计划项目(2020ZDLSF05-01)。
关键词
野生林麝
宏基因组学
消化道微生物
抗生素抗性基因
差异富集分析
wild forest musk deer
metagenomics
digestive tract microbiota
antibiotic resistance genes
differential enrichment analysis
