摘要
目的探讨玻璃化解冻(vitrificative thawing,VT)和慢速解冻(slow thawing,ST)对程序化冷冻胚胎解冻移植的影响,优化程序化冷冻胚胎的解冻方案,从而提高不孕不育临床治疗效果。方法选取2016年1月至2018年12月解放军第九六〇医院生殖医学中心行解冻胚胎移植的400例患者,使用随机数分配的方式(Excel数据随机分组),分为VT组(200例)和ST组(200例),比较两组患者最初施行体外受精-胚胎移植(invitro fertilization-embryo transfer,IVF-ET)时的基本情况、胚胎发育情况及本次移植胚胎复苏情况、移植情况和临床妊娠结局。结果两组患者年龄、不孕年限、获卵数、促性腺激素(gonadotropins,Gn)用量和基础促卵泡成熟激素(follicle stimulating hormone,FSH)值等基本情况比较,差异无统计学意义(P>0.05),两组MⅡ卵率(metaphase II egg rate)、受精率、卵裂率、优质胚胎率、可用胚胎率和冷冻胚胎率比较,差异无统计学意义(P>0.05)。但VT组的胚胎复苏完整率和复苏可用率(88.34%、97.51%)明显高于ST组(72.47%、82.23%),差异有统计学意义(P<0.001);两组患者胚胎移植日移植次数、移植胚胎数和内膜厚度比较,差异无统计学意义(P>0.05);两组临床妊娠结局比较,差异无统计学意义(P>0.05)。结论VT可以显著提高程序化冷冻胚胎的复苏效果,提高程序化冷冻胚胎患者的胚胎利用率,可以考虑成为ST的较好替代方案。
Objective To discuss the influence of vitrificative thawing(VT)and slow thawing(ST)on programmed frozen embryo thawing transfer,optimize the thawing program of programmed frozen embryo,and improve the clinical treatment effect of infertility.Methods From January 2016 to December 2018,400 patients who underwent thawing embryo transfer at Reproductive Medicine Center,the 960th Hospital of the PLA Joint Logistics Support Force were selected and divided into VT groups(200 cases)and ST group(200 cases)(randomized by Excel data)using random number assignment,compare the basic conditions,embryo development and embryo recovery and transfer situation and clinical pregnancy outcome of the two groups during the initial invitro fertilization-embryo transfer(IVF-ET).Results There were no statistically significant differences between the two groups of patients in basic conditions such as age,years of infertility,number of eggs obtained,gonadotropins(Gn)dosage and basic follicle stimulating hormone(FSH)values(P>0.05).There were no significant difference between the two groups of MII egg rate(metaphase II egg rate),fertilization rate,cleavage rate,high-quality embryo rate,usable embryo rate and frozen embryo rate(P>0.05).However,the embryo recovery complete rate and recovery availability rate(88.34%,97.51%)of the VT group were significantly higher than those of the ST group(72.47%,82.23%),and the differences were statistically significant(P<0.001).There were no statistically significant difference in the number of transfers,the number of embryos transferred and the thickness of the intima on the day of embryo transfer(P>0.05).There was no significant difference in the clinical pregnancy outcome between the two groups(P>0.05).Conclusion VT can significantly improve the recovery effect of programmed frozen embryos and increase the embryo utilization rate of patients with programmed frozen embryos.It can be considered as a better alternative to ST.
作者
王建业
唐宁
吕丹
WANG Jianye;TANG Ning;LYU Dan(Reproductive Medicine Center,No.1 Medicine Center of the General Hospital of PLA,Beijing 100700;Reproductive Medicine Center,the 960th Hospital of the PLA Joint Logistics Support Force,Jinan Shandong 250031;Pain Management Department of Tianjin First Center Hospital,Tianjin 300192,P.R.China)
出处
《中国计划生育和妇产科》
2021年第1期71-73,78,共4页
Chinese Journal of Family Planning & Gynecotokology
关键词
玻璃化解冻
程序化冷冻
慢速解冻
胚胎移植
vitrification and thawing
programmed freezing
slow thawing
embryo transfer
