摘要
异养小球藻体积小、发酵液成分复杂,导致藻细胞采收困难、采收成本高。通过比较三氯化铁、硫酸铝、壳聚糖、聚丙烯酰胺、霉菌5种絮凝剂,并利用单因素试验、响应面分析对三氯化铁絮凝法收集异养小球藻进行工艺条件优化,获得了二次回归模型。得到了最优变量为三氯化铁添加量2.0 g·L^-1、搅拌速率150 r·min^-1、搅拌时间20 min,在此条件下,异养小球藻采收率达到95.3%,收集成本为0.07元·kg^-1。采收率高、采收成本低,为工业化应用奠定了基础。
The small volume of heterotrophic Chlorella and the complex components of fermentation broth make it difficult in algae cell harvesting and high recovery cost.In this study,five flocculant of ferric chloride,aluminum sulfate,chitosan,polyacrylamide and mold were compared,single factor test and response surface analysis were used to optimize the process conditions of collecting heterotrophic Chlorella by ferric chloride flocculation method,and a quadratic regression model was obtained.The optimal variables were the dosage of ferric chloride 2.0 g·L^-1,agitation rate 150 r·min^-1,agitation time 20 min.Under this condition,the recovery rate reached 95.3%,and the collection cost was 0.07 yuan·kg^-1.The recovery rate was high and the recovery cost was low,which laid the foundation for the industrialization application.
作者
黄伟伟
毕生雷
杨迪
刘雨
辛纳慧
李钰媛
张乃群
HUANG Weiwei;BI Shenglei;YANG Di;LIU Yu;XIN Nahui;LI Yuyuan;ZHANG Naiqun(College of Life Science and Technology,Nanyang Normal University,Henan Nanyang 473061,China;State Key Laboratory of Motor Vehicle Biofuel Technology,Henan Tianguan Group Co.,Ltd.,Henan Nanyang 473003,China;Nanyang Vocational College of Agriculture,Henan Nanyang 473000,China)
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2021年第1期186-193,共8页
Journal of Agricultural Science and Technology
基金
河南省科技攻关计划项目(162102310259)
南阳师范学院研究生创新基金项目(STP2017028)。
关键词
异养小球藻
絮凝剂
采收率
响应面分析
heterotrophic Chlorella
flocculant
recovery rate
response surface analysis
